Acid bone lysates reduce bone regeneration in rat calvaria defects
Author
dc.contributor.author
Strauss Avendaño, Franz Josef
Author
dc.contributor.author
Kuchler, Ulrike
Author
dc.contributor.author
Kobatake, Reiko
Author
dc.contributor.author
Heimel, Patrick
Author
dc.contributor.author
Tangl, Stefan
Author
dc.contributor.author
Gruber, Reinhard
Admission date
dc.date.accessioned
2020-10-14T00:41:08Z
Available date
dc.date.available
2020-10-14T00:41:08Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
J Biomed Mater Res. 2020;1–7
es_ES
Identifier
dc.identifier.other
10.1002/jbm.a.37050
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/177116
Abstract
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Acid bone lysates (ABLs) represent the growth factors and other molecules released during autologous graft resorption. However, the impact of these bone-derived growth factors on the healing of bone defects has not yet been investigated. The aim of the present study was, therefore, to examine the impact of ABLs adsorbed to collagen membranes on bone regeneration. To this end, in 16 female Sprague Dawley rats, a standardized 5-mm-diameter critical size defect on the calvarial bone was created. The defects were covered with collagen membranes that had been soaked either in serum-free media or ABLs followed by lyophilization. After a healing period of 4 weeks, micro-computed tomography (mu CT) and histological analyses by means of undecalcified thin ground sections were performed. mu CT analysis of the inner 4 mm of the calvaria defect showed a greater bone defect coverage in the control group when compared to ABL group, 29.8% (confidence interval [CI]: 17.7-50.3) versus 5.6% (CI: 1.0-29.8,p= .03), respectively. Moreover, we found significantly more absolute bone volume (BV) in the control group when compared to ABL group, 0.59 mm(3)(CI: 0.27-1.25) versus 0.07 mm(3)(CI: 0.06-0.59,p= .04), respectively. Histomorphometry confirmed these findings with a relative BV in the central compartment of 14.1% (CI: 8.4-20.6) versus 5.6% (CI: 3.4-7.9,p= .004), respectively. These findings indicate that bone-derived growth factors contained in ABLs are able to attenuate bone regeneration within collagen membranes.
es_ES
Patrocinador
dc.description.sponsorship
Osteology Foundation
17-125
17-219
Austrian Science Fund (FWF)
4072-B28