Browsing by Author "XX577768"

Now showing items 21-40 of 40

    • Ion channels in a skeletal muscle cell line from a Duchenne muscular dystrophy patient 
      Caviedes Codelia, Raúl; Caviedes Fernández, Pablo; Liberona Leppe, José; Jaimovich Pérez, Enrique (John Wiley and Sons, 1994)
      A cell line (RCDMD), derived from a muscle biopsy taken from a 7-year-old patient with Duchenne muscular dystrophy (DMD), was established in vitro using conditioned media from the UCHT1 thyroid cell line as described ...
    • Isolation of Viable Porcine Islets by Selective Osmotic Shock Without Enzymatic Digestion 
      Atwater Ransom, Illani; Guajardo, M.; Caviedes Fernández, Pablo; Jeffs, S.; Parrau, D.; Valencia, M.; Romero, C.; Arriagada Abarzúa, Christian; Caamaño, Edgardo; Salas, A.; Olguín, F.; Atlagich, Miljenko; Maas, R.; Mears, David; Rojas, Eduardo (ELSEVIER, 2010)
      Islet transplantation is a potential cure for type 1 diabetes, but clinical results have been disappointing. Currently, islet isolation is by enzymatic digestion of the pancreas which has significant pitfalls: warm ...
    • Knockdown of amyloid precursor protein normalizes cholinergic function in a cell line derived from the ... 
      Opazo, Patricia; Saud, Katherine; Saint Pierre, Michelle de; Cárdenas, Ana María; Allen, David D.; Segura Aguilar, Juan; Caviedes Codelia, Raúl; Caviedes Fernández, Pablo (WILEY-LISS, 2006-11-01)
      We have generated immortal neuronal cell lines from normal and trisomy 16 (Ts16) mice, a model for Down syndrome (DS). Ts16 lines overexpress DS-related genes (App, amyloid precursor protein; Sod1, Cu/Zn superoxide dismutase) ...
    • Knockdown of Myo-Inositol transporter SMIT1 normalizes cholinergic and glutamatergic function in an ... 
      Cárdenas, Ana María; Fernández Olivares, Paola; Díaz Franulic, Ignacio; González Jamett, Arlek M.; Shimahara, Takeshi; Segura Aguilar, Juan; Caviedes Codelia, Raúl; Caviedes Fernández, Pablo (Springer, 2017)
      The Na+/myo-inositol cotransporter (SMIT1) is overexpressed in human Down syndrome (DS) and in trisomy 16 fetal mice (Ts16), an animal model of the human condition. SMIT1 overexpression determines increased levels of ...
    • Microencapsulación de células y tejido para terapia celular 
      Alvo Vergara, Andrés; Neira Carrillo, Andrónico; Caviedes Fernández, Pablo; Gac Espinoza, Patricio; Cabané Toledo, Patricio (SOC CIRUJANOS CHILE, 2011-02)
      Microencapsulation is a technique that protects viable cells in semi-permeable membranes, which allow passage of essential molecules while stopping larger molecules, such as antibodies, involved in the death of transplanted ...
    • Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells ... 
      Jara, Claudia; Oyarzún Ampuero, Felipe; Carrión, Flavio; González Echeverría, Esteban; Cappelli, Claudio; Caviedes Fernández, Pablo (BMC, 2020)
      Background: In type I diabetes mellitus (T1DM) pancreatic beta cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet ...
    • Monoamine transporter inhibitors and norepinephrine reduce dopamine-dependent iron toxicity in cells derived ... 
      Paris Pizarro, Irmgard; Martínez Alvarado, Pedro; Pérez Pastene, Carolina; Vieira, Marcelo N.N.; Olea Azar, Claudio; Raisman Vozari, Rita; Cárdenas, Sergio; Graumann, Rebecca; Caviedes Fernández, Pablo; Segura Aguilar, Juan (BLACKWELL PUBLISHING LTD, 2005-03)
      The role of dopamine in iron uptake into catecholaminergic neurons, and dopamine oxidation to aminochrome and its one-electron reduction in iron-mediated neurotoxicity, was studied in RCSN-3 cells, which express both ...
    • Myofibers deficient in connexins 43 and 45 expression protect mice from skeletal muscle and systemic ... 
      Fernández, Gabriela; Arias Bravo, Guisselle; Bevilacqua, Jorge A.; Castillo Ruíz, Mario; Caviedes Fernández, Pablo; Sáez, Juan C.; Cea, Luis A. (Elsevier, 2020)
      Dysferlinopathy is a genetic human disease caused by mutations in the gene that encodes the dysferlin protein (DYSF). Dysferlin is believed to play a relevant role in cell membrane repair. However, in dysferlin-deficient ...
    • Neuronal dysfunction in Down syndrome: Contribution of neuronal models in cell culture 
      Saud, Katherine; Arriagada Abarzúa, Christian; Cárdenas, Ana María; Shimahara, Takeshi; Allen, David D.; Caviedes Codelia, Raúl; Caviedes Fernández, Pablo (ELSEVIER, 2006-05)
      Down syndrome (DS) in humans, or trisomy of autosome 21, represents the hyperdiploidy that most frequently survives gestation, reaching an incidence of I in 700 live births. The condition is associated with multisystemic ...
    • Neurotoxicity of some MAO Inhibitors in Adult Rat Hypothalamic Cell Culture 
      Hurtado Guzmán, C.; Martínez Alvarado, P.; Dagnino Subiabre, A.; Paris Pizarro, Irmgard; Caviedes Fernández, Pablo; Caviedes Codelia, Raúl; Cassels Niven, Bruce; Segura Aguilar, Juan (Taylor & Francis Ltd, 2001-05-04)
      Monoamine oxidase-A (MAO-A) (amiflamine (AMF) and 4-methylthioamphetamine (MTA)) and MAO-B (Ldeprenyl) inhibitors were found to be cytotoxic in a concentration-dependent manner for RCHTcells derived from adult rat ...
    • On the neurotoxicity mechanism of leukoaminochrome o-semiquinone radical derived from dopamine oxidation: ... 
      Arriagada, C.; Paris Pizarro, Irmgard; Matas, M. J. S. de las; Martínez Alvarado, P.; Cárdenas, S.; Castañeda, P.; Graumann, Rebecca; Pérez Pastene, C.; Olea Azar, Claudio; Couve, E.; Herrero, M. T.; Caviedes Fernández, Pablo; Segura Aguilar, Juan (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2004-07)
      Leukoaminochrome o-semiquinone radical is generated during one-electron reduction of dopamine oxidation product aminochrome when DT-diaphorase is inhibited. Incubation of 100 muM aminochrome with 100 muM dicoumarol, an ...
    • Optimización de cultivos de hepatocitos humanos para estudios de citotoxicidad 
      Cabané Toledo, Patricio; Díaz Jeraldo, Juan; Rojas C., Jorge; Maluenda G., Fernando; Rencoret, Guillermo; Saud, Katherine; Godoy V., M. Loreto; Ibacache A., Daniela; Ledezma S., Alejandra; Caviedes Codelia, Raúl; Caviedes Fernández, Pablo (2007-04)
      Los cultivos de hepatocitos entregan un valioso acercamiento al estudio de las funciones metabólicas específicas del hígado, evaluación de citotoxicidad. No existen líneas humanas inmortales con función normal. La ...
    • Osseointegration properties of titanium dental implants modifiedwith a nanostructured coating based on ... 
      Covarrubias Gallardo, Cristián; Mattmann, Matías; Marttens Castro, Alfredo von; Caviedes Fernández, Pablo; Arriagada, Cristián; Valenzuela, Francisco; Rodríguez Vives, Juan Pablo; Corral Núñez, Camila (Elsevier, 2016)
      The fabrication of a nanoporous silica coating loaded with bioactive glass nanoparticles (nBG/NSC) on titanium dental implant surface and its in vitro and in vivo evaluation is presented. The coating was produced by a ...
    • Poly(lactic acid) composites based on graphene oxide particles with antibacterial behavior enhanced by ... 
      Arriagada, Paulo; Palza Cordero, Humberto; Palma Fluxá, Patricia Liliana; Flores Carrasco, Marcos; Caviedes Fernández, Pablo (Wiley, 2018)
      Poly(lactic acid) (PLA) is a biodegradable and biocompatible polyester widely used in biomedical applications. Unfortunately, this biomaterial suffers from some shortcomings related with the absence of both bioactivity and ...
    • Possible role of salsolinol quinone methide in the decrease of RCSN-3 cell survival 
      Martínez Alvarado, P.; Dagnino Subiabre, Alexies; Paris Pizarro, Irmgard; Metodiewa, D.; Welch, C. J.; Olea Azar, Claudio; Caviedes Fernández, Pablo; Caviedes Codelia, Raúl; Segura Aguilar, Juan (ACADEMIC PRESS INC, 2001-05-25)
      The endogenous dopamine-derived neurotoxin salsolinol was found to decrease survival in the dopaminergic neuronal cell line RCSN-3, derived from adult rat substantia nigra in a concentration-dependent manner (208 muM ...
    • Producción recombinante de péptidos con potencial terapéutico en Escherichia coli 
      Lascani Monsalve, Jorge Andrés (Universidad de Chile, 2014)
      Durante las últimas décadas, una rama de la investigación biotecnológica se ha enfocado en desarrollar y optimizar procesos de producción de proteínas recombinantes orientadas a aplicaciones terapéuticas. En particular, ...
    • Proteome Profiling and Ultrastructural Characterization of the Human RCMH Cell Line: Myoblastic Properties ... 
      Kollipara, Laxmikanth; Buchkremer, Stephan; Weis, Joachim; Brauers, Eva; Hoss, Mareike; Ruetten, Stephan; Caviedes Fernández, Pablo; Zahedi, René P.; Roos, Andreas (AMER CHEMICAL SOC., 2016)
      Studying (neuro)muscular disorders is a major topic in biomedicine with a demand for suitable model systems. Continuous cell culture (in vitro) systems have several technical advantages over in vivo systems and became ...
    • Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line 
      Paula Lima, Andrea; Arriagada Abarzúa, Christian; Toro Sánchez, Rodrigo; Cárdenas, Ana María; Caviedes Codelia, Raúl; Ferreira, Sergio T.; Caviedes Fernández, Pablo (SOC BIOLGIA CHILE, 2008)
      We have previously characterized a number of small molecule organic compounds that prevent the aggregation of the β-amyloid peptide and its neurotoxicity in hippocampal neuronal cultures. We have now evaluated the effects ...
    • Sol–gel synthesis and in vitro bioactivity of copper and zinc-doped silicate bioactive glasses and glass-ceramics 
      Bejarano Narváez, Julián; Caviedes Fernández, Pablo; Palza Cordero, Humberto (IOP, 2015)
      Metal doping of bioactive glasses based on ternary 60SiO2–36CaO–4P2O5 (58S) and quaternary 60SiO2–25CaO–11Na2O–4P2O5 (NaBG) mol% compositions synthesized using a sol–gel process was analyzed. In particular, the effect ...
    • The absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes 
      Cea Pisani, Luis Andrés; Bevilacqua, Jorge; Arriagada Abarzúa, Christian; Cárdenas, Ana María; Bigot, Anne; Mouly, Vincent; Sáez, Juan C.; Caviedes Fernández, Pablo (2016)
      Background: Mutations in the gene encoding for dysferlin cause recessive autosomal muscular dystrophies called dysferlinopathies. These mutations induce several alterations in skeletal muscles, including, inflammation, ...