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Authordc.contributor.authorHebeisen, Simon 
Authordc.contributor.authorHeidtmann, Hannelore es_CL
Authordc.contributor.authorCosmelli, Diego es_CL
Authordc.contributor.authorGonzález, Carlos es_CL
Authordc.contributor.authorPoser, Barbara es_CL
Authordc.contributor.authorLatorre, Ramón es_CL
Authordc.contributor.authorÁlvarez Araya, Osvaldo es_CL
Authordc.contributor.authorFahlke, Christoph es_CL
Admission datedc.date.accessioned2008-03-26T11:19:36Z
Available datedc.date.available2008-03-26T11:19:36Z
Publication datedc.date.issued2003-04
Cita de ítemdc.identifier.citationBIOPHYSICAL JOURNAL Volume: 84 Issue: 4 Pages: 2306-2318 Published: APR 2003en
Identifierdc.identifier.issn0006-3495
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/118658
Abstractdc.description.abstractCIC-4 and CIC-5 are mammalian CIC isoforms with unique ion conduction and gating properties. Macroscopic current recordings in heterologous expression systems revealed very small currents at negative potentials, whereas a substantially larger instantaneous current amplitude and a subsequent activation wore observed upon depolarization. Neither the functional basis nor the physiological impact of these channel features are currently understood. Here, we used whole-cell recordings to study pore properties of human CIC-4 channels heterologously expressed in tsA201 or HEK293 cells. Variance analysis demonstrated that the prominent rectification of the instantaneous macroscopic current amplitude is due to a voltage-dependent unitary current conductance. The single channel amplitudes are very small, i.e., 0.10 +/- 0.02 pA at +140 mV for external Cl- and internal I-. Conductivity and permeability sequences were determined for various external and internal anions, and both values increase for anions with lower dehydration energies. CIC-4 exhibits pore properties that are distinct from other CIC isoforms. These differences can be explained by assuming differences in the size of the pore narrowing and the electrostatic potentials within the ion conduction pathways.en
Lenguagedc.language.isoenen
Publisherdc.publisherBIOPHYSICAL SOCIETYen
Keywordsdc.subjectMUSCLE CHLORIDE CHANNELen
Títulodc.titleAnion permeation in human CIC-4 channelsen
Document typedc.typeArtículo de revista


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