Simultaneous determination of acetaminophen and tramadol by second derivative spectrophotometry
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2008-06Metadata
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Toral Ponce, María
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Simultaneous determination of acetaminophen and tramadol by second derivative spectrophotometry
Abstract
A rapid method for the simultaneous determination of acetaminophen (A) and tramadol (T) by second derivative spectrophotometric has been developed.
From a solvent effect studies and the spectral behaviours of A and T, ethanol was selected as solvent. For a Δλ value of 210 nm a smoothing factor of 8,000
and scale factor of 1,000,000 were selected, because in these conditions the signal/noise ratio was favoured in order to avoid error. In these conditions also it is
possible the simultaneous determination of A/T in a molar relation of 17/1 contained in pharmaceutical formulations. At 285.7 nm the second derivative value is T
concentration dependent, corresponding to zero-crossing point of A. On the other hand, T does not absorb between 296.0 to 400.0 nm, thus 308.0 nm was selected
for A determination by graphic method. The determination ranges for A and T were 8.1x10-7 - 51x10-5 mol/L and 3.4 x10-7 - 5.0 x10-5 mol/L, respectively and can
be determined with good precision and accuracy, without previous separation. A study of interferents, was carried out by using the excipients Zafin® tablet. The
recoveries were 97.4 ± 2.0% and 100.6 ± 1.7% for A and T, respectively. This tablet containing both drugs was assayed using the methods developed, showing a
good accuracy and precision.
Patrocinador
The authors are grateful to the National Fund for Development of Sciences
and Technology (FONDECYT), project 1070905 and DIUC project Nº
204021021-1 for the financial support and to Laboratorio Saval S.A. Santiago,
Chile, for his help providing acetaminophen and tramadol.
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JOURNAL OF THE CHILEAN CHEMICAL SOCIETY, Volume: 53, Issue: 2, Pages: 1543-1547, 2008
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