All-trans retinoic acid mediates enhanced T reg cell growth, differentiation, and gut homing in the face of high levels of co-stimulation

Abstract

We demonstrate that all-trans retinoic acid (RA) induces FoxP3 adaptive T regulatory cells (A-Tregs) to acquire a gut-homing phenotype ( 4 7 CC chemokine receptor 9 ) and the capacity to home to the lamina propria of the small intestine. Under conditions that favor the differentiation of A-Tregs (transforming growth factor – 1 and interleukin 2) in vitro, the inclusion of RA induces nearly all activated CD4 T cells to express FoxP3 and greatly increases the accumulation of these cells. In the absence of RA, A-Treg differentiation is abruptly impaired by profi cient antigen presenting cells or through direct co-stimulation. In the presence of RA, A-Treg generation occurs even in the presence of high levels of co-stimulation, with RA attenuating co-stimulation from interfering from FoxP3 induction. The recognition that RA induces gut imprinting, together with our fi nding that it enhances A-Treg conversion, differentiation, and expansion, indicates that RA production in vivo may drive both the imprinting and A-Treg development in the face of overt infl ammation.