Gibberellin biosynthesis and gibberellin oxidase activities in Fusarium sacchari, Fusarium konzum and Fusarium subglutinans strains
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2010-05-02Metadata
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Troncoso, Claudia
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Gibberellin biosynthesis and gibberellin oxidase activities in Fusarium sacchari, Fusarium konzum and Fusarium subglutinans strains
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Abstract
Several isolates of three Fusarium species associated with the Gibberella fujikuroi species complex were
characterized for their ability to synthesize gibberellins (GAs): Fusarium sacchari (mating population
B), Fusarium konzum (mating population I) and Fusarium subglutinans (mating population E). Of these,
F. sacchari is phylogenetically related to Fusarium fujikuroi and is grouped in the Asian clade of the complex,
while F. konzum and F. subglutinans are only distantly related to Fusarium fujikuroi and belong to the
American clade. Variability was found between the different F. sacchari strains tested. Five isolates
(B-12756; B-1732, B-7610, B-1721 and B-1797) were active in GA biosynthesis and accumulated GA3
in the culture fluid (2.76–28.4 lg/mL), while two others (B-3828 and B-1725) were inactive. GA3 levels
in strain B-12756 increased by 2.9 times upon complementation with ggs2 and cps-ks genes from
F. fujikuroi. Of six F. konzum isolates tested, three (I-10653; I-11616; I-11893) synthesized GAs, mainly
GA1, at a low level (less than 0.1 lg/mL). Non-producing F. konzum strains contained no GA oxidase activities
as found for the two F. subglutinans strains tested. These results indicate that the ability to produce
GAs is present in other species of the G. fujikuroi complex beside F. fujikuroi, but might differ significantly
in different isolates of the same species.
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This work was supported by Fondo Nacional de Ciencia y Tecnología
(Grant 1061127), Deutsche Forschungsgemeinschaft
(DFG) (Tu 101/9-5) and by the CONICYT/DFG Cooperation Program
(Project 105-2007).
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PHYTOCHEMISTRY, Volume: 71, Issue: 11-12, Pages: 1322-1331, 2010
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