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Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans

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2012
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Ravanal, María Cristina
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Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans
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Author
  • Ravanal, María Cristina;
  • Espinosa, Yeison;
  • Rosa, Lorena;
  • Vaca Cerezo, Inmaculada;
  • Polanco, Rubén;
  • Eyzaguirre, Jaime;
  • Levicán, Gloria;
  • Chávez, Renato;
Abstract
The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde- 3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.
Patrocinador
This work was supported by grants from DICYT- USACH, FONDECYT 1100084 and UNAB DI-03-10/R. I. Vaca acknowledges support from the ‘‘Programa Bicentenario de Ciencia y Tecnologı´a’’ program (Chile), project PDA13.
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URI: https://repositorio.uchile.cl/handle/2250/119392
DOI: DOI 10.1007/s10267-011-0144-1
Quote Item
Mycoscience (2012) 53:152–155
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