Abnormal Glycosphingolipid Mannosylation Triggers Salicylic Acid-Mediated Responses in Arabidopsis
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2013-05Metadata
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Mortimer, Jenny C.
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Abnormal Glycosphingolipid Mannosylation Triggers Salicylic Acid-Mediated Responses in Arabidopsis
Author
- Mortimer, Jenny C.;
- Yu, Xiaolan;
- Albrecht, Sandra;
- Sicilia, Francesca;
- Huichalaf, Mariela;
- Ampuero, Diego;
- Michaelson, Louise V.;
- Murphy, Alex M.;
- Matsunaga, Toshiro;
- Kurz, Samantha;
- Stephens, Elaine;
- Baldwin, Timothy C.;
- Ishii, Tadashi;
- Napier, Johnathan A.;
- Weber, Andreas P.M.;
- Handford, Michael;
- Dupree, Paul;
Abstract
The Arabidopsis thaliana protein GOLGI-LOCALIZED NUCLEOTIDE SUGAR TRANSPORTER (GONST1) has been previously identified as a GDP-D-mannose transporter. It has been hypothesized that GONST1 provides precursors for the synthesis of cell wall polysaccharides, such as glucomannan. Here, we show that in vitro GONST1 can transport all four plant GDP-sugars. However, gonst1 mutants have no reduction in glucomannan quantity and show no detectable alterations in other cell wall polysaccharides. By contrast, we show that a class of glycosylated sphingolipids (glycosylinositol phosphoceramides [GIPCs]) contains Man and that this mannosylation is affected in gonst1. GONST1 therefore is a Golgi GDP-sugar transporter that specifically supplies GDP-Man to the Golgi lumen for GIPC synthesis. gonst1 plants have a dwarfed phenotype and a constitutive hypersensitive response with elevated salicylic acid levels. This suggests an unexpected role for GIPC sugar decorations in sphingolipid function and plant defense signaling. Additionally, we discuss these data in the context of substrate channeling within the Golgi.
General note
Artículo de publicación ISI.
Patrocinador
Biotechnology and Biological Sciences Research Council
Grants BB/D010446/1 and BB/G016240/1, as well as funding from the
European Community’s Seventh Framework Programme (FP7/2007-
2013) under Grant Agreement 211982 (RENEWALL) (to P.D.). The work
was also supported by Fondecyt Grants 11060470 and 1100129 (to
M.H.). A.P.M.W. appreciates funding by Deutsche Forschungsgemeinschaft
Grant WE 2231/6-1. Rothamsted Research receives grant-aided
support from the Biotechnology and Biological Sciences Research
Council.
Quote Item
The Plant Cell, Vol. 25: 1881–1894, May 2013
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