Simultaneous detection of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus using real-time PCR and high resolution melting analysis
Author
dc.contributor.author
Komorowska, Beata
Author
dc.contributor.author
Fiore, Nicola
es_CL
Author
dc.contributor.author
Zamorano Carrasco, Alan
es_CL
Author
dc.contributor.author
Li, Ruhui
es_CL
Admission date
dc.date.accessioned
2014-12-24T14:31:03Z
Available date
dc.date.available
2014-12-24T14:31:03Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
Molecular and Cellular Probes 28 (2014) 186-191
en_US
Identifier
dc.identifier.other
dx.doi.org/10.1016/j.mcp.2014.03.002
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/120262
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
In this study, the real-time PCR assays were combined with high resolution melting (HRM) analysis for
the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle
virus (CGRMV) infection in sweet cherry trees. Detection of CNRMV and CGRMV was performed in a realtime
PCR using a primer set for both of them or duplex real-time PCR that included one specific primer
set for each virus. These two strategies allowed us to confirmed virus infection in all tested samples. In 17
field samples the technique revealed samples positive for CNRMV or CGRMV as well as positive for both
viruses. In addition, the HRM analysis made it possible to differentiate clearly between CNRMV and
CGRMV. Sequence variations among CNRMV and CGRMV isolates observed from the HRM peaks were
confirmed by sequencing. To test the capability to use this method in field, forty one sweet cherry
samples were examined by HRM analysis. The HRM data showed that seven samples were positive for
CNRMV and three were infected with CGRMV. The results presented in this study indicated that realtime
PCR followed by HRM analysis provides sensitive, automated and rapid tool to detect and differentiate
between CNRMV and CGRMV isolates.
en_US
Patrocinador
dc.description.sponsorship
This work was financially supported by project N N310 116738
from the National Science Center Poland, Poland.
Simultaneous detection of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus using real-time PCR and high resolution melting analysis