Development of Gold Standard Ion-Selective Electrode-Based Methods for Fluoride Analysis
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2011Metadata
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Martínez Mier, E. A.
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Development of Gold Standard Ion-Selective Electrode-Based Methods for Fluoride Analysis
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Abstract
Background/Aims: Currently available techniques for fluoride
analysis are not standardized. Therefore, this study was
designed to develop standardized methods for analyzing
fluoride in biological and nonbiological samples used for
dental research. Methods: A group of nine laboratories analyzed
a set of standardized samples for fluoride concentration
using their own methods. The group then reviewed existing
analytical techniques for fluoride analysis, identified
inconsistencies in the use of these techniques and conducted
testing to resolve differences. Based on the results of the
testing undertaken to define the best approaches for the
analysis, the group developed recommendations for direct
and microdiffusion methods using the fluoride ion-selective
electrode. Results: Initial results demonstrated that there
was no consensus regarding the choice of analytical techniques
for different types of samples. Although for several types of samples, the results of the fluoride analyses were
similar among some laboratories, greater differences were
observed for saliva, food and beverage samples. In spite of
these initial differences, precise and true values of fluoride
concentration, as well as smaller differences between laboratories,
were obtained once the standardized methodologies
were used. Intraclass correlation coefficients ranged
from 0.90 to 0.93, for the analysis of a certified reference material,
using the standardized methodologies. Conclusion:
The results of this study demonstrate that the development
and use of standardized protocols for F analysis significantly
decreased differences among laboratories and resulted in
more precise and true values.
Patrocinador
Study supported by a National Institute of Dental and Craniofacial
Research grant (NIDCR grant R21 DE 14716-1).
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Caries Res 2011;45:3–12
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