Effects of High Iron and Glucose Concentrations over the Relative Expression of Bcl2, Bax, and Mfn2 in MIN6 Cells
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2013Metadata
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Fuentes, Marcela
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Effects of High Iron and Glucose Concentrations over the Relative Expression of Bcl2, Bax, and Mfn2 in MIN6 Cells
Abstract
Type 2 diabetes is characterized by hyperglycemia
and oxidative stress. Hyperglycemia is linked to mitochondrial
dysfunction and reduced β-cell mass due to the
reduced expression of genes such as Mfn2 as well as the
participation of the Bcl2 gene family, responsible for increased
apoptosis. The purpose of this study was to describe
the effect of different iron and/or glucose concentrations
over Mfn2, Bax, and Bcl2 expressions in a β-pancreatic
cell line (MIN6 cells). MIN6 cells were pre-incubated with
different iron and/or glucose concentrations, and the relative
mRNA abundance of the Bcl2/Bax ratio and of Mfn2 genes
was measured by qRT-PCR. Heme oxygenase (HO) activity,
iron uptake, superoxide dismutase activity, and glutathione
content were also determined. The Bcl2/Bax ratio increased
and Mfn2 expression decreased in MIN6 cells after glucose
stimulation. These effects were higher when glucose and
iron were incubated together. Additionally, treatment with
glucose/iron showed a higher HO activity. Our study revealed
that high glucose/Fe concentrations in MIN6 cells
induced an increase of the Bcl2/Bax ratio, an indicator of
increased cell apoptosis.
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Artículo de publicación ISI
Patrocinador
Fondo Nacional de Desarrollo Científico y Tecnológico de Chile
(FONDECYT, grant no. 1085173) to MA
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URI: https://repositorio.uchile.cl/handle/2250/124073
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Biol Trace Elem Res (2013) 153:390–395 DOI 10.1007/s12011-013-9666-z
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