About
Contact
Help
Sending publications
How to publish
Advanced Search
View Item 
  •   Home
  • Facultad de Medicina
  • Artículos de revistas
  • View Item
  •   Home
  • Facultad de Medicina
  • Artículos de revistas
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse byCommunities and CollectionsDateAuthorsTitlesSubjectsThis CollectionDateAuthorsTitlesSubjects

My Account

Login to my accountRegister
Biblioteca Digital - Universidad de Chile
Revistas Chilenas
Repositorios Latinoamericanos
Tesis LatinoAmericanas
Tesis chilenas
Related linksRegistry of Open Access RepositoriesOpenDOARGoogle scholarCOREBASE
My Account
Login to my accountRegister

Tetracycline resistance in Chilean clinical isolates of Helicobacter pylori

Artículo
Thumbnail
Open/Download
IconToledo_Hector.pdf (130.7Kb)
Publication date
2010
Metadata
Show full item record
Cómo citar
Toledo Araya, Héctor
Cómo citar
Tetracycline resistance in Chilean clinical isolates of Helicobacter pylori
.
Copiar
Cerrar

Author
  • Toledo Araya, Héctor;
  • López Solís, Remigio;
Abstract
Objectives: Since high-level tetracycline resistance in Helicobacter pylori has been associated with a AGA926– 928!TTC substitution in the 16S rRNA genes rrnA/B, the aim of the study was to screen for tetracycline resistance in H. pylori clinical isolates obtained from Santiago, Chile by using a recently reported molecular assay. Methods: A PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the conserved 535 bp region of the H. pylori 16S rRNA genes rrnA/B (between nucleotides 710 and 1245) using HinfI was followed by DNA sequencing of the same fragment obtained from tetracycline-resistant H. pylori clinical isolates. Results: The PCR-RFLP assay revealed that the tetracycline-resistant H. pylori isolates lacked the AGA926– 928!TTC substitution. In contrast, DNA sequencing of the 535 bp PCR fragment from 11 tetracycline-resistant H. pylori Chilean clinical isolates showed an association of low-level tetracycline resistance with 1 bp (A928C) or 2 bp (AG926–927!GT and/or A926G/A928C) substitutions in both 16S rRNA genes. Conclusions: The PCR-RFLP (HinfI) assay alone is unreliable for the detection of tetracycline resistance in Chilean clinical isolates of H. pylori. To that end, it must be complemented by sequencing of the 535 bp PCR fragment.
Patrocinador
This research was partly supported by grants AMAYOR 02/4-2 from DID-University of Chile, Enlace ICBM-FM-UCh 2006 and Fondecyt 1085193 (H. T.).
Identifier
URI: https://repositorio.uchile.cl/handle/2250/128727
DOI: doi:10.1093/jac/dkp457
Quote Item
J Antimicrob Chemother 2010; 65: 470–473
Collections
  • Artículos de revistas
xmlui.footer.title
31 participating institutions
More than 73,000 publications
More than 110,000 topics
More than 75,000 authors
Published in the repository
  • How to publish
  • Definitions
  • Copyright
  • Frequent questions
Documents
  • Dating Guide
  • Thesis authorization
  • Document authorization
  • How to prepare a thesis (PDF)
Services
  • Digital library
  • Chilean academic journals portal
  • Latin American Repository Network
  • Latin American theses
  • Chilean theses
Dirección de Servicios de Información y Bibliotecas (SISIB)
Universidad de Chile

© 2020 DSpace
  • Access my account