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Estudio de reproducibilidad del examen de cuantificación de VIH-1 por la técnica Nuclisens HIV-1 QT® en muestras de plasma de pacientes infectados con VIH-1

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2002
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Muñoz Gómez, María Gabriela
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Estudio de reproducibilidad del examen de cuantificación de VIH-1 por la técnica Nuclisens HIV-1 QT® en muestras de plasma de pacientes infectados con VIH-1
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Author
  • Muñoz Gómez, María Gabriela;
  • Venegas Santos, Mauricio;
Abstract
The viral load quantification for Human Immunodeficiency Virus in infected patients has been proved to be a valuable test as a good predictor for infection progression and antiviral treatment response. The reproducibility of a new viral load assay, Nuclisens HIV-1 QTTM, was assesed by comparing different volume plasma samples in 11 HIV positive patients. Each sample was tested at least twice, in the same or different runs. A total of 29 tests were done. The best reproducibility was obtained when using an initial input equal or greater than 500 μl of plasma, with a precision rate of 0.18 and a coefficient of variation of 20%. The intra and inter - assay variation was 0.19 and 0.07, respectively. The good reproducibility found in this study, the easy performance and the wide linear range of quantification makes this assay fully recommended as a routine test for HIV viral load in HIV-1 infected patients.
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URI: https://repositorio.uchile.cl/handle/2250/129068
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Rev Chil Infect (2002); 19 (1): 25-31
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