Proteomic analysis of a segregant population reveals candidate proteins linked to mealiness in peach
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2016Metadata
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Miyasaka Almeida, Andréa
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Proteomic analysis of a segregant population reveals candidate proteins linked to mealiness in peach
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Abstract
Peaches are stored at low temperatures to delay ripening and increase postharvest life. However some varieties are susceptible to chilling injury, which leads to fruit mealiness, browning and flesh bleeding. In order to identify potential markers associated with chilling injury, we performed proteomic analyses on a segregating population with contrasting susceptibility to chilling-induced mealiness. Chilling-induced mealiness was assessed by measuring juiciness in fruits that have been stored in cold and then allowed to ripen. Fruit mesocarp and leaf proteome from contrasting segregants were analyzed using 2-DE gels. Comparison of protein abundance between segregants revealed 133 spots from fruit mesocarp and 36 from leaf. Thirty four fruit mesocarp proteins were identified from these spots. Most of these proteins were related to ethylene synthesis, ABA response and stress response. Leaf protein analyses identified 22 proteins, most of which related to energy metabolism. Some of the genes that code for these proteins have been previously correlated with chilling injury through transcript analyses and co-segregation with mealiness QTLs. The results from this study, further deciphers the molecular mechanisms associated with chilling response in peach fruit, and identifies candidate proteins linked to mealiness in peach which may be used as putative markers for this trait.
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Artículo de publicación ISI
Patrocinador
FONDAP CRG
15090007
Genoma-Chile FONDEF
G0711001
FONDECYT
1130197
Basal program
PFB-16
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URI: https://repositorio.uchile.cl/handle/2250/136609
DOI: DOI: 10.1016/j.jprot.2015.10.011
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Journal of Proteomics 131 (2016) 71–81
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