Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence
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Handford, Michael
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Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence
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Nucleotide sugar transporters (NSTs) are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function(s) of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis (Arabidopsis thaliana (L.) Heynh.), which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein (GFP) tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carri
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URI: https://repositorio.uchile.cl/handle/2250/153898
DOI: 10.1093/mp/sss074
ISSN: 17529867
16742052
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Molecular Plant, Volumen 5, Issue 6, 2018, Pages 1263-1280
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