Interaction between glutathione and ampicillin: an a.c. polarographic method

Abstract

The interaction of glutathione with ampicillin was investigated by a new a.c. polarographic procedure. Ampicillin was found to interact strongly with glutathione. The stability constants were ascertained from the polarographic data, and the free energy change, ΔGo, the enthalpy change, ΔHo, and the entropy change, ΔSo, of the interaction were computed from the data at different temperatures. The introduction of the polarographic method to the study of interactions between molecules of biological significance (other than metal-ion complexes), and the possibility of GSH binding to ampicillin in vivo are the principal contributions of this work