Hydroxyl radical activation of a Ca2+-sensitive nonselective cation channel involved in epithelial cell necrosis
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2004Metadata
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Simon, Felipe
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Hydroxyl radical activation of a Ca2+-sensitive nonselective cation channel involved in epithelial cell necrosis
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Abstract
In a previous work the involvement of a fenamate-sensitive Ca 2+-activated nonselective cation channel (NSCC) in free radical-induced rat liver cell necrosis was demonstrated (5). Therefore, we studied the effect of radical oxygen species and oxidizing agents on the gating behavior of a NSCC in a liver-derived epithelial cell line (HTC). Single-channel currents were recorded in HTC cells by the excised inside-out configuration of the patch-clamp technique. In this cell line, we characterize a 19-pS Ca 2+-activated, ATP- and fenamate-sensitive NSCC nearly equally permeable to monovalent cations. In the presence of Fe2+, exposure of the intracellular side of NSCC to H2O2 increased their open probability (Po) by ∼40% without affecting the unitary conductance. Desferrioxamine as well as the hydroxyl radical (.OH) scavenger MCI-186 inhibited the effect of H2O2, indicating that the increase in Po was mediated by .OH. Exposure of the patch membrane to the oxidizing agent 5,5′-dithio-bis-2-nit
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URI: https://repositorio.uchile.cl/handle/2250/164546
DOI: 10.1152/ajpcell.00041.2004
ISSN: 03636143
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American Journal of Physiology - Cell Physiology, Volumen 287, Issue 4 56-4, 2018,
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