Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products
Artículo
Open/ Download
Publication date
2011Metadata
Show full item record
Cómo citar
Mattison, Kirsten
Cómo citar
Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products
Author
Abstract
Noroviruses are implicated in many worldwide institutional, food and waterborne outbreaks each year. Genetic typing of isolates is valuable for monitoring outbreak spread as well as variation in circulating strains. Microarrays have the potential to provide rapid genotype information for norovirus samples. The NoroChip v3.0 provides an oligonucleotide hybridization platform to screen for over 600 potential interactions in each experiment. The NoroChip v3.0 was developed at Health Canada and validated in seven international partner laboratories. Each laboratory validated the NoroChip v3.0 using norovirus amplicons routinely characterized in their testing protocols. Fragments from the capsid region (region C) and a 2.4. kb amplicon spanning polymerase and capsid sequences (region AD) were validated in six of the partner laboratories and provided correct genogroup typing information (GI or GII) when hybridized to the NoroChip v3.0. Results indicate that the current limiting factor for imp
Indexation
Artículo de publicación SCOPUS
Identifier
URI: https://repositorio.uchile.cl/handle/2250/165211
DOI: 10.1016/j.jviromet.2011.02.011
ISSN: 01660934
Quote Item
Journal of Virological Methods, Volumen 173, Issue 2, 2018, Pages 233-250
Collections