About
Contact
Help
Sending publications
How to publish
Advanced Search
View Item 
  •   Home
  • Facultad de Medicina
  • Artículos de revistas
  • View Item
  •   Home
  • Facultad de Medicina
  • Artículos de revistas
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse byCommunities and CollectionsDateAuthorsTitlesSubjectsThis CollectionDateAuthorsTitlesSubjects

My Account

Login to my accountRegister
Biblioteca Digital - Universidad de Chile
Revistas Chilenas
Repositorios Latinoamericanos
Tesis LatinoAmericanas
Tesis chilenas
Related linksRegistry of Open Access RepositoriesOpenDOARGoogle scholarCOREBASE
My Account
Login to my accountRegister

Lipid droplets are both highly oxidized and Plin2-covered in hepatocytes of diet-induced obese mice

Artículo
Thumbnail
Open/Download
IconLipid-droplets.pdf (649.5Kb)
Access note
Acceso a solo metadatos
Publication date
2020
Metadata
Show full item record
Cómo citar
Nocetti, Diego
Cómo citar
Lipid droplets are both highly oxidized and Plin2-covered in hepatocytes of diet-induced obese mice
.
Copiar
Cerrar

Author
  • Nocetti, Diego;
  • Espinosa, Alejandra;
  • Pino de la Fuente, Francisco;
  • Sacristán, Camila;
  • Bucarey, José Luis;
  • Ruiz, Paulina;
  • Valenzuela, Rodrigo;
  • Chouinard Watkins, Raphael;
  • Pepper Bergholz, Inés;
  • Troncoso, Rodrigo;
  • Puente Díaz, Luis;
Abstract
Chronic high-fat diet feeding is associated with obesity and accumulation of fat in the liver, leading to the development of insulin resistance and nonalcoholic fatty liver disease. This condition is characterized by the presence of a high number of intrahepatic lipid droplets (LDs), with changes in the perilipin pattern covering them. This work aimed to describe the distribution of perilipin (Plin) 2, an LD-associated protein involved in neutral lipid storage, and Pliny, which favors lipid oxidation in LD, and to evaluate lipid peroxidation through live-cell visualization using the lipophilic fluorescent probe C11-BODIPY581/591 in fresh hepatocytes isolated from mice fed a high-fat diet (HFD). Male C57B1/6J adult mice were divided into control and HFD groups and fed with a control diet (10% fat, 20% protein, and 70% carbohydrates) or an HFD (60% fat, 20% protein, and 20% carbohydrates) for 8 weeks. The animals fed the HFD showed a significant increase of Plin2 in LD of hepatocytes. LD from HFD-fed mice have a stronger lipid peroxidation level than control hepatocytes. These data provide evidence that obesity status is accompanied by a higher degree of lipid peroxidation in hepatocytes, both in the cytoplasm and in the fats stored inside the LD.
Indexation
Artículo de publicación ISI
 
Artículo de publicación SCOPUS
 
Identifier
URI: https://repositorio.uchile.cl/handle/2250/179867
DOI: 10.1139/apnm-2019-0966
Quote Item
Applied Physiology Nutrition and Metabolism Volumen: 45 Número: 12 Páginas: 1368-1376 Dec 2020
Collections
  • Artículos de revistas
xmlui.footer.title
31 participating institutions
More than 73,000 publications
More than 110,000 topics
More than 75,000 authors
Published in the repository
  • How to publish
  • Definitions
  • Copyright
  • Frequent questions
Documents
  • Dating Guide
  • Thesis authorization
  • Document authorization
  • How to prepare a thesis (PDF)
Services
  • Digital library
  • Chilean academic journals portal
  • Latin American Repository Network
  • Latin American theses
  • Chilean theses
Dirección de Servicios de Información y Bibliotecas (SISIB)
Universidad de Chile

© 2020 DSpace
  • Access my account