| Author | dc.contributor.author | Acevedo Acevedo, Mónica | |
| Author | dc.contributor.author | Arbildua, José J. | es_CL |
| Author | dc.contributor.author | Monasterio Opazo, Octavio | es_CL |
| Author | dc.contributor.author | Toledo Araya, Héctor | es_CL |
| Author | dc.contributor.author | León Decap, Oscar | es_CL |
| Admission date | dc.date.accessioned | 2010-06-14T18:58:03Z | |
| Available date | dc.date.available | 2010-06-14T18:58:03Z | |
| Publication date | dc.date.issued | 2010 | |
| Cita de ítem | dc.identifier.citation | Archives of Biochemistry and Biophysics 495 (2010) 28–34 | en_US |
| Identifier | dc.identifier.other | doi:10.1016/j.abb.2009.12.018 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128544 | |
| Abstract | dc.description.abstract | X-ray diffraction data on a few retroviral integrases show a flexible loop near the active site. By sequence
alignment, the peptide region 207–218 of Mo-MLV IN appears to correspond to this flexible loop. In this
study, residues H208, Y211, R212, Q214, S215 and S216 of Mo-MLV IN were mutated to determine their
role on enzyme activity. We found that Y211A, R212A, R212K and Q214A decreased integration activity,
while disintegration and 30-processing were not significantly affected. By contrast H208A was completely
inactive in all the assays. The core domain of Mo-MLV integrase was modeled and the flexibility of the
region 207–216 was analyzed. Substitutions with low integration activity showed a lower flexibility than
wild type integrase. We propose that the peptide region 207–216 is a flexible loop and that H208, Y211,
R212 and Q214 of this loop are involved in the correct assembly of the DNA-integrase complex during
integration. | en_US |
| Patrocinador | dc.description.sponsorship | This work was funded by grant Fondecyt 1040409. | en_US |
| Lenguage | dc.language.iso | en | en_US |
| Publisher | dc.publisher | ELSEVIER | en_US |
| Keywords | dc.subject | Integrase | en_US |
| Título | dc.title | Role of the 207–218 peptide region of Moloney murine leukemia virus integrase in enzyme catalysis | en_US |
| Document type | dc.type | Artículo de revista | |
