MicroRNAs miR-21a and miR-93 are down regulated in peripheral blood mononuclear cells (PBMCs) from patients with type 1 diabetes
Author
dc.contributor.author
Salas Pérez, Francisca
Author
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Codner Dujovne, Ethel
es_CL
Author
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Valencia, Elizabeth
es_CL
Author
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Pizarro, Carolina
es_CL
Author
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Carrasco, Elena
es_CL
Author
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Pérez Bravo, Francisco
es_CL
Admission date
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2014-02-11T19:27:08Z
Available date
dc.date.available
2014-02-11T19:27:08Z
Publication date
dc.date.issued
2013
Cita de ítem
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Immunobiology 218 (2013) 733– 737
en_US
Identifier
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doi 10.1016/j.imbio.2012.08.276
Identifier
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https://repositorio.uchile.cl/handle/2250/129277
General note
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Artículo de publicación ISI
en_US
Abstract
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Introduction: It is well established that type 1 diabetes (T1D) is an autoimmune disease. Controversial data
exists regarding the differential control of the immune system in T1D patients compared to unaffected
individuals. MicroRNAs (miRNAs) are involved in the control of gene expression (by negative regulation
of gene expression at post-transcriptional level, by mediating translational repression or degradation of
the mRNA targets). Their potential role in T cell activation and autoimmunity is controversial.
Aim: We investigated the expression profile of miR-21a and miR-93 in PMC samples of 20 T1D patients
and 20 healthy controls by means of qPCR in different glucose concentrations (basal, 11 nM and 25 mM),
and we analyzed the possible relationship of this expression pattern with autoimmunity.
Results: MiR-21a was significantly underexpressed in T1D samples (media values expression 0.23 ± 0.05,
p < 0.01) compared to controls (values less than 1 indicate a decrease in gene expression). When the PMCs
were incubated with glucose 11 mM and 25 mM, miR-21a expression decreased in controls and increased
in T1D samples (0.506 ± 0.05, p < 0.04). MiR-93 was underexpressed in T1D patients (0.331 ± 0.05, p < 0.02)
compared to control samples. However, when the PBMCs were incubated with glucose, no changes were
observed. No association with autoimmunity was observed.
Conclusion: We demonstrated that miRNAs have a differential expression in PBMCs from T1D patients
compared to controls, suggesting that these miRNAs or others could be involved in T cell regulation.