Steroid Anti-Inflammatory Effects Did Not Improve Organ Quality in Brain-Dead Rats
Author
dc.contributor.author
Rebolledo, Rolando
Author
dc.contributor.author
Liu, Bo
Author
dc.contributor.author
Akhtar, Mohammed
Author
dc.contributor.author
Ottens, Petra
Author
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Zhang, Jian-ning
Author
dc.contributor.author
Ploeg, Rutger
Author
dc.contributor.author
Leuvenink, Henri
Admission date
dc.date.accessioned
2015-08-04T15:38:44Z
Available date
dc.date.available
2015-08-04T15:38:44Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
BioMed Research International Volume 2015, Article ID 207534, 10 pages
en_US
Identifier
dc.identifier.issn
2314-6141
Identifier
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doi: 10.1155/2015/207534
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/132332
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
Effect of glucocorticoid administration on improving the outcomes of kidney and liver allografts has not been clearly elucidated.
This study investigated the effect of prednisolone administration after onset of brain death (BD) on kidney and liver in a controlled
rat model of BD. BD was induced in rats by inflating an epidurally placed balloon catheter. Animals were treated with saline
or prednisolone (5, 12.5, or 22.5 mg/kg) one hour after the onset of BD. After 4 hours of BD, experiments were terminated and
serum and tissues were collected. Tissue gene and protein expression were measured for markers of inflammation, apoptosis, and
cellular stress response markers. Prednisolone caused a reduction of plasma levels of IL-6, while the tissue expression of IL-6, IL-
1𝛽, and MCP-1 in both kidney and liver were also reduced. Creatinine plasma levels, complement (C3) expression, HSP-70, HO-1,
Bcl2/BAX ratio, and PMNinflux did not significantly change in kidney nor liver. Plasma AST and LDH levels were increased in the
prednisolone treated group. Our results demonstrate prednisolone can has an anti-inflammatory effectmediated through reducing
serum circulating cytokines. However, this anti-inflammatory effect does not translate into improved kidney function and indeed
was associated with increased liver injury markers.