Angiotensin II directly impairs adipogenic differentiation of human preadipose cells
Author
dc.contributor.author
Palominos, Marisol M.
Author
dc.contributor.author
Dunner, Natalia H.
Author
dc.contributor.author
Wabitsch, Martin
Author
dc.contributor.author
Rojas, Cecilia V.
Admission date
dc.date.accessioned
2015-12-08T02:14:41Z
Available date
dc.date.available
2015-12-08T02:14:41Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
Mol Cell Biochem (2015) 408:115–122
en_US
Identifier
dc.identifier.other
DOI 10.1007/s11010-015-2487-y
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/135507
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
Angiotensin II reduces adipogenic differentiation
of preadipose cells present in the stroma-vascular
fraction of human adipose tissue, which also includes
several cell types. Because of the ability of non-adipose
lineage cells in the stroma-vascular fraction to respond to
angiotensin II, it is not possible to unequivocally ascribe
the anti-adipogenic response to a direct effect of this hormone
on preadipose cells. Therefore, we used the human
Simpson–Golabi–Behmel syndrome (SGBS) preadipocyte
cell strain to investigate the consequences of angiotensin II
treatment on adipogenic differentiation under serum-free
conditions, by assessing expression of typical adipocyte
markers perilipin and fatty acid-binding protein 4
(FABP4), at the transcript and protein level. Reverse
transcription-polymerase chain reaction showed that perilipin
and FABP4 transcripts were, respectively, reduced to
0.33 ± 0.07 (P\0.05) and 0.41 ± 0.19-fold (P\0.05)
in SGBS cells induced to adipogenic differentiation in the
presence of angiotensin II. Western Blot analysis corroborated
reduction of the corresponding proteins to
0.23 ± 0.21 (P\0.01) and 0.46 ± 0.30-fold (P\0.01)
the respective controls without angiotensin II. Angiotensin II also impaired morphological changes associated with
early adipogenesis. Hence, we demonstrated that angiotensin
II is able to directly reduce adipogenic differentiation
of SGBS preadipose cells.