Helicobacter pylori Infection Is Associated with Decreased Expression of SLC5A8, a Cancer Suppressor Gene, in Young Children
Author
dc.contributor.author
Orellana Manzano, Andrea
Author
dc.contributor.author
O'Ryan Gallardo, Miguel
Author
dc.contributor.author
Lagomarcino, Anne
Author
dc.contributor.author
George Carreño, Sergio
Author
dc.contributor.author
Muñoz, Mindy
Author
dc.contributor.author
Mamani Manzano, Nora
Author
dc.contributor.author
Serrano, Carolina
Author
dc.contributor.author
Harris, Paul
Author
dc.contributor.author
Ramilo, Octavio
Author
dc.contributor.author
Mejías, Asunción
Author
dc.contributor.author
Torres Torretti, Juan Pablo
Author
dc.contributor.author
Lucero Álvarez, Yalda
Author
dc.contributor.author
Quest, Andrew F. G.
Admission date
dc.date.accessioned
2017-03-23T19:39:57Z
Available date
dc.date.available
2017-03-23T19:39:57Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
Frontiers in Cellular and Infection Microbiology October 2016 Volume 6 Article 121
es_ES
Identifier
dc.identifier.other
10.3389/fcimb.2016.00121
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/143274
Abstract
dc.description.abstract
Background: Helicobacter pylori infects half of the world's population and causes gastric cancer in a subset of infected adults. Previous blood microarray findings showed that apparently healthy children, persistently infected with H. pylori have differential gene expression compared to age-matched, non-infected children. SLC5A8, a cancer suppressor gene with decreased expression among infected children, was chosen for further study based on bioinformatics analysis.
Methods: A pilot study was conducted using specific qRT-PCR amplification of SLC5A8 in blood samples from H. pylori infected and non-infected children, followed by a larger, blinded, case-control study. We then analyzed gastric tissue from H. pylori infected and non-infected children undergoing endoscopy for clinical purposes.
Results: Demographics, clinical findings, and family history were similar between groups. SLC5A8 expression was decreased in infected vs. non infected children in blood, 0.12 (IQR: 0-0.89) vs. 1.86 (IQR: 0-8.94, P = 0.002), and in gastric tissue, 0.08 (IQR: 0.04-0.15) vs. 1.88 (IQR: 0.55-2.56; P = 0.001). Children who were both stool positive and seropositive for H. pylori had the lowest SLC5A8 expression levels.
Conclusions: H. pylori infection is associated with suppression of SCL5A8, a cancer suppressor gene, in both blood and tissue samples from young children
es_ES
Patrocinador
dc.description.sponsorship
Fondo Nacional de Desarrollo Cientifico y Tecnologico 1061079 1100514 1130387 11140232
Comision Nacional de Investigacion Cientifica y Tecnologica 21140520
Fondo de Financiamiento de Centros de Investigation en Areas Prioritarias 15130011
Secretarial de Educacion Superior, Ciencia, Tecnologia e Innovacion