Detection of Helicobacter pylori by Real-Time PCR for 16s rRNA in Stools of NonInfected Healthy Children, Using ELISA Antigen Stool Test as the Gold Standard
Author
dc.contributor.author
George, Sergio
Author
dc.contributor.author
Mamani Manzano, Nora
Author
dc.contributor.author
Lucero Álvarez, Yalda
Author
dc.contributor.author
Torres Torretti, Juan Pablo
Author
dc.contributor.author
Farfán Urzúa, Mauricio
Author
dc.contributor.author
Lagomarcino, Anne J.
Author
dc.contributor.author
Orellana, Andrea
Author
dc.contributor.author
O'Ryan Gallardo, Miguel
Admission date
dc.date.accessioned
2017-11-23T15:05:12Z
Available date
dc.date.available
2017-11-23T15:05:12Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
Helicobacter 21: 606–612 Dec 2016
es_ES
Identifier
dc.identifier.other
10.1111/hel.12318
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/145779
Abstract
dc.description.abstract
BackgroundWe previously detected Helicobacter pylori infection by stool antigen ELISA assay in 33-41% of asymptomatic Chilean children between 2-3 years of age, of which 11-20% had a transient infection and 21-22% a persistent infection. A total of 88% of ELISA-positive samples were also rtPCR positive, while 37/133 (33%) of ELISA-negative stool samples were rtPCR positive. The significance of a ELISA-negative/rtPCR-positive sample requires clarification. We aimed to determine whether rtPCR is able to detect persistent infections not detected by ELISA.
Materials and MethodsWe selected 36 children with an ELISA-negative/rtPCR-positive stool sample, of which 25 were never H. pylori infected according to ELISA, and 11 had a transient infection with an ELISA-positive sample before or after the discordant sample. At least two additional consecutive ELISA-negative samples per child were tested in duplicate by rtPCR for the 16s rRNA gene.
ResultsA total of 14 of 78 (17.9%) rtPCR reactions were positive, but only 4/78 (5.1%) were positive in both duplicates, representing a total of 3/36 (8.3%) children with an additional rtPCR-positive sample, only one of whom was persistently negative by ELISA. One child with a transient infection had two positive rtPCR reactions despite negative ELISA samples.
ConclusionsIn H. pylori noninfected or transiently infected children, as determined by stool ELISA, additional ELISA-negative/rtPCR-positive stool samples were found in 8.3% of children, but a possible persistent infection was only identified in 2.7% of children. Thus, the characterization of infection dynamics in children is not being misrepresented by application of stool ELISA. Furthermore, rtPCR does not significantly improve dynamic characterization.
Detection of Helicobacter pylori by Real-Time PCR for 16s rRNA in Stools of NonInfected Healthy Children, Using ELISA Antigen Stool Test as the Gold Standard