Author | dc.contributor.author | González Mañán, Daniel | |
Author | dc.contributor.author | D'Espessailles Tapia, Amanda | |
Author | dc.contributor.author | Dossi Muñoz, Camila G. | |
Author | dc.contributor.author | San Martín, Marcela | |
Author | dc.contributor.author | Mancilla, Rodrigo A. | |
Author | dc.contributor.author | Tapia Opazo, Gladys S. | |
Admission date | dc.date.accessioned | 2018-03-26T13:14:23Z | |
Available date | dc.date.available | 2018-03-26T13:14:23Z | |
Publication date | dc.date.issued | 2017 | |
Cita de ítem | dc.identifier.citation | Journal of Nutrition Volumen: 147 Número: 4 Páginas: 579-588 (2017) | es_ES |
Identifier | dc.identifier.other | 10.3945/jn.116.243261 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/146988 | |
Abstract | dc.description.abstract | Background: Rosa mosqueta (RM) oil is characterized by high concentrations of antioxidants and alpha-linolenic acid (ALA; 18: 3n-3). We have previously demonstrated in male C57BL/6J mice that RM decreases hepatic steatosis, a condition strongly associated with oxidative stress and inflammation.
Objective: We studied the molecular mechanisms that underlie the role of RM in preventing high-fat diet (HFD)-induced oxidative stress and inflammation.
Methods: Male C57BL/6J mice aged 28 d and weighing 12-14 g were divided into the following groups and fed for 12 wk: control diet (CD; 10% fat, 20% protein, and 70% carbohydrates); CD + RM (1.94 mg ALA . g body weight(-1) . d(-1) administered by oral gavage); HFD (60% fat, 20% protein, and 20% carbohydrates); and HFD + RM. General parameters (body weight, visceral fat, and histology); glucose metabolism [homeostasis model assessment and blood glucose area under the curve (AUC)]; oxidative stress [hepatic nuclear factor (erythroid-derived 2)-like-2 (NRF2) and heme oxygenase 1 (HO-1) concentrations]; and inflammation [hepatic peroxisome proliferator-activated receptor alpha (PPAR-alpha) and acylcoenzyme A oxidase 1 (ACOX1) concentrations, blood tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) concentrations, and Tnfa and Il1b mRNA expression in liver and visceral adipose tissue] were evaluated.
Results: In the HFD + RM mice, the final body weight (24.8 +/- 1.1 g) was 19% lower than in the HFD mice (30.+/- 6 2.8 g) (P < 0.05). Visceral fat was 34% lower in the HFD + RM mice than in the HFD mice (P < 0.05). The blood glucose AUC was 29% lower and Tnfa and Il1b expression levels were 47% and 59% lower, respectively, in the HFD + RM mice than in the HFD mice (P < 0.05). HFD + RM mice had 40% less hepatic steatosis (P < 0.05) and lower upregulation of PPAR-alpha (33%), ACOX1 (50%), NRF2 (39%), and HO-1 (68%) protein concentrations than did the HFD mice (P < 0.05).
Conclusions: Our findings suggest that RM supplementation prevents the obese phenotype observed in HFD-fed mice by downregulating inflammatory cytokine expression and secretion and stimulating hepatic antioxidant and fatty acid oxidation markers. | es_ES |
Patrocinador | dc.description.sponsorship | National Fund for Scientific and Technological Development
1140547 | es_ES |
Lenguage | dc.language.iso | en | es_ES |
Publisher | dc.publisher | American Society for Nutrition | es_ES |
Source | dc.source | Journal of Nutrition | es_ES |
Keywords | dc.subject | Rosa mosqueta | es_ES |
Keywords | dc.subject | Inflammation | es_ES |
Keywords | dc.subject | Oxidative stress | es_ES |
Keywords | dc.subject | PPAR-alpha | es_ES |
Keywords | dc.subject | NRF2 | es_ES |
Título | dc.title | Rosa mosqueta oil prevents oxidative stress and inflammation through the upregulation of PPAR-alpha and NRF2 in C57BL/6J mice fed a high-fat diet | es_ES |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso abierto | es_ES |
Cataloguer | uchile.catalogador | pgv | es_ES |
Indexation | uchile.index | Artículo de publicación ISI | es_ES |