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Ex vivo infection of human placental chorionic villi explants with Trypanosoma cruzi and Toxoplasma gondii induces different Toll-like receptor expression and cytokine/chemokine profiles

Authordc.contributor.authorCastillo Rivas, Christian 
Authordc.contributor.authorMuñoz Villanueva, Lorena 
Authordc.contributor.authorCarrillo Werner, Ileana 
Authordc.contributor.authorLiempi Manquel, Ana 
Authordc.contributor.authorGallardo Arce, Christian 
Authordc.contributor.authorGalanti Garrone, Norbel 
Authordc.contributor.authorMaya Arango, Juan 
Authordc.contributor.authorKemmerling Weis, Ulrike 
Admission datedc.date.accessioned2018-04-06T18:18:17Z
Available datedc.date.available2018-04-06T18:18:17Z
Publication datedc.date.issued2017-07
Cita de ítemdc.identifier.citationAm J Reprod Immunol. 2017;78:e12660.es_ES
Identifierdc.identifier.other10.1111/aji.12660
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/147186
Abstractdc.description.abstractProblemTrypanosoma cruzi and Toxoplasma gondii present, respectively, low and high congenital transmission rates. The placenta as an immune regulatory organ expresses TLRs, leading to the secretion of cytokines. Both parasites are recognized by TLR-2, TLR-4, and TLR-9. Here, we studied if the parasites induce differences in TLR protein expression, cytokine profiles, and whether receptor inhibition is related to parasite infection. Method of studyPlacental tissue explants were infected ex vivo with each parasite, TLRs protein expression, cytokine profile and parasite infection were determined by Western blotting, ELISA and qPCR. ResultsTrypanosoma cruzi and Toxoplasma gondii infection is related to TLR-2 and TLR-4/TLR-9, respectively. Trypanosoma cruzi elicits an increase in TNF-, IL-1, IL-6, IL-8 and IL-10 cytokine secretion whereas T.gondii only increases the secretion of IL-8. ConclusionThe susceptibility of the placenta to each parasite is mediated partially by the innate immune response.es_ES
Patrocinadordc.description.sponsorshipERANET-LAC ELAC2014/HID-0328 ENL027/16 UREDES URC-024/16 Fondo Nacional de Desarrollo Científico y Tecnológico 1130189 1130113es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherWileyes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceAmerican Journal of Reproductive Immunologyes_ES
Keywordsdc.subjectCytokineses_ES
Keywordsdc.subjectPlacental infectiones_ES
Keywordsdc.subjectToll-like receptorses_ES
Keywordsdc.subjectToxoplasma gondiies_ES
Keywordsdc.subjectTrypanosoma cruzies_ES
Títulodc.titleEx vivo infection of human placental chorionic villi explants with Trypanosoma cruzi and Toxoplasma gondii induces different Toll-like receptor expression and cytokine/chemokine profileses_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorpgves_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile