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Old yellow enzyme from Trypanosoma cruzi exhibits in vivo prostaglandin F-2 alpha synthase activity and has a key role in parasite infection and drug susceptibility

Authordc.contributor.authorDíaz Viraque, Florencia 
Authordc.contributor.authorChiribao, María Laura 
Authordc.contributor.authorTrochine, Andrea 
Authordc.contributor.authorGonzález Herrera, Fabiola 
Authordc.contributor.authorCastillo, Christian 
Authordc.contributor.authorLiempi, Ana 
Authordc.contributor.authorKemmerling Weis, Ulrike 
Authordc.contributor.authorDiego Maya, Juan 
Authordc.contributor.authorRobello, Carlos 
Cita de ítemdc.identifier.citationFront. Immunol. 9:456es_ES
Abstractdc.description.abstractThe discovery that trypanosomatids, unicellular organisms of the order Kinetoplastida, are capable of synthesizing prostaglandins raised questions about the role of these molecules during parasitic infections. Multiple studies indicate that prostaglandins could be related to the infection processes and pathogenesis in trypanosomatids. This work aimed to unveil the role of the prostaglandin F-2 alpha synthase TcOYE in the establishment of Trypanosoma cruzi infection, the causative agent of Chagas disease. This chronic disease affects several million people in Latin America causing high morbidity and mortality. Here, we propose a prokaryotic evolutionary origin for TcOYE, and then we used in vitro and in vivo experiments to show that T. cruzi prostaglandin F-2 alpha synthase plays an important role in modulating the infection process. TcOYE overexpressing parasites were less able to complete the infective cycle in cell culture infections and increased cardiac tissue parasitic load in infected mice. Additionally, parasites overexpressing the enzyme increased PGF(2)alpha synthesis from arachidonic acid. Finally, an increase in benznidazole and nifurtimox susceptibility in TcOYE overexpressing parasites showed its participation in activating the currently anti-chagasic drugs, which added to its observed ability to confer resistance to hydrogen peroxide, highlights the relevance of this enzyme in multiple events including host-parasite interaction.es_ES
Patrocinadordc.description.sponsorshipFOCEM (Mercosur Structural Convergence Fund) COF 03/11 ANII (Agencia Nacional de Investigacion e Innovacion-Uruguay) POS_NAC_2014_1_102168es_ES
Publisherdc.publisherFrontiers media SAes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers in Immunologyes_ES
Keywordsdc.subjectTrypanosoma cruzies_ES
Keywordsdc.subjectProstaglandin F-2 alpha synthasees_ES
Keywordsdc.subjectOld Yellow Enzymees_ES
Keywordsdc.subjectDifferentially expressed genees_ES
Keywordsdc.subjectBenznidazol and nifurtimox activationes_ES
Títulodc.titleOld yellow enzyme from Trypanosoma cruzi exhibits in vivo prostaglandin F-2 alpha synthase activity and has a key role in parasite infection and drug susceptibilityes_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile