Proanthocyanidin-containing polyphenol extracts from fruits prevent the inhibitory effect of hydrogen sulfide on human colonocyte oxygen consumption
Author
dc.contributor.author
Andriamihaja, Mireille
Author
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Lan, Annaig
Author
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Beaumont, Martin
Author
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Grauso, Marta
Author
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Gotteland, Martin
Author
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Pastene, Edgar
Author
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Cires, María José
Author
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Carrasco Pozo, Catalina
Author
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Tome, Daniel
Author
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Blachier, Francois
Admission date
dc.date.accessioned
2018-10-08T15:56:03Z
Available date
dc.date.available
2018-10-08T15:56:03Z
Publication date
dc.date.issued
2018-06
Cita de ítem
dc.identifier.citation
Amino Acids (2018) 50:755–763
es_ES
Identifier
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10.1007/s00726-018-2558-y
Identifier
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https://repositorio.uchile.cl/handle/2250/152016
Abstract
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Hydrogen sulfide (H2S), a metabolic end product synthesized by the microbiota from l-cysteine, has been shown to act at low micromolar concentration as a mineral oxidative substrate in colonocytes while acting as an inhibitor of oxygen consumption at higher luminal concentrations (65 mu M and above). From the previous works showing that polyphenols can bind volatile sulfur compounds, we hypothesized that different dietary proanthocyanidin-containing polyphenol (PACs) plant extracts might modulate the inhibitory effect of H2S on colonocyte respiration. Using the model of human HT-29 Glc-/+ cell colonocytes, we show here that pre-incubation of 65 mu M of the H2S donor NaHS with the different polyphenol extracts markedly reduced the inhibitory effect of NaHS on colonocyte oxygen consumption. Our studies on HT-29 Glc-/+ cell respiration performed in the absence or the presence of PACs reveal rapid binding of H2S with the sulfide-oxidizing unit and slower binding of H2S to the cytochrome c oxidase (complex IV of the respiratory chain). Despite acute inhibition of colonocyte respiration, no measurable effect of NaHS on paracellular permeability was recorded after 24 h treatment using the Caco-2 colonocyte monolayer model. The results are discussed in the context of the binding of excessive bacterial metabolites by unabsorbed dietary compounds and of the capacity of colonocytes to adapt to changing luminal environment.
es_ES
Patrocinador
dc.description.sponsorship
Evaluation-Orientation de la Cooperation Scientifique
C12S01
Fondecyt
1120290
AgroParisTech
INRA
Universite Paris-Saclay
University of Chile in Santiago
University of Concepcion