Purification and preliminary sequencing of Tc45, an immunodominant Trypanosoma cruzi antigen: Absence of homology with cruzipain, cruzain, and a 46-kilodalton protein
Author
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Aguillón Gutiérrez, Juan Carlos
Author
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Bustos,
Author
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Vallejos, Carla
Author
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Hermosilla, Manuel
Author
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Morello Casté, Antonio
Author
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Repetto Scaramelli, Yolanda
Author
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Hellman,
Author
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Orn,
Author
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Ferreira e Ferreira, Efigenia
Admission date
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2019-01-29T14:55:17Z
Available date
dc.date.available
2019-01-29T14:55:17Z
Publication date
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1995
Cita de ítem
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American Journal of Tropical Medicine and Hygiene, Volumen 53, Issue 2, 2018, Pages 211-215
Identifier
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00029637
Identifier
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10.4269/ajtmh.1995.53.211
Identifier
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https://repositorio.uchile.cl/handle/2250/161329
Abstract
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In spite of being separated by more than 20 million years of evolution, the murine and human immune systems share extensive similarities. Thus, experimental results obtained with the murine model may have predictive value for human Chagas' disease. Challenge of the H-2 congenic mouse strains A.SW (H-2(s)) and A.CA (H-2(f)) with Trypanosoma cruzi yields different results. The A.CA animals die approximately 12 days postinfection, while A.SW mice survive indefinitely. A 45-kD protein (Tc45), an antigen differentially recognized by the A.SW strain, is present in cultured epimastigotes and blood trypomastigotes. We describe here its purification from epimastigotes. The presence of Tc45 was monitored and a single band was detected. Since the molecular weights of Tc45, cruzipain, cruzain, and a 46-kD parasite polypeptide are similar, it was important to determine if these molecules are related. A complete lack of homology was observed when the sequences of cruzain, cruzipain, and the 46-kD po
Purification and preliminary sequencing of Tc45, an immunodominant Trypanosoma cruzi antigen: Absence of homology with cruzipain, cruzain, and a 46-kilodalton protein