Comparison of immunofluorescence, enzyme immunoassay and cell culture for the diagnosis of Chlamydia trachomatis in urogenital infections Comparación de la inmunofluorescencia, ensayo inmunoenzimático y cultivo celular para el diagnóstico de Chlamydia tra
Author
dc.contributor.author
Martínez Martínez, Regina
Author
dc.contributor.author
Pinto Pinto, Andrea Alejandra
Author
dc.contributor.author
Gaggero Brillouet, Aldo
Author
dc.contributor.author
Arroyave,
Admission date
dc.date.accessioned
2019-01-29T15:47:33Z
Available date
dc.date.available
2019-01-29T15:47:33Z
Publication date
dc.date.issued
1991
Cita de ítem
dc.identifier.citation
Revista medica de Chile, Volumen 119, Issue 2, 2018, Pages 164-168
Identifier
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00349887
Identifier
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https://repositorio.uchile.cl/handle/2250/162401
Abstract
dc.description.abstract
We evaluated the usefulness of the direct immunofluorescence test with monoclonal antibodies and the enzyme immunoassay in comparison with isolation in cell cultures for the diagnosis of Chlamydia trachomatis in 55 endocervical specimens from female prostitutes and 21 urethral specimens from men with diagnosis of nongonococcal urethritis. In comparison with culture, the enzyme immunoassay had a sensitivity of 100% and a specificity of 95%. The immunofluorescence test had a sensitivity of 92% and a specificity of 98%. The positive and negative predictive values for the enzyme immunoassay were 81% and 100% and for immunofluorescence 92% and 98% respectively. The immunologic methods appear to be satisfactory alternatives to culture for detecting C trachomatis in genital specimens in the studied populations.
Comparison of immunofluorescence, enzyme immunoassay and cell culture for the diagnosis of Chlamydia trachomatis in urogenital infections Comparación de la inmunofluorescencia, ensayo inmunoenzimático y cultivo celular para el diagnóstico de Chlamydia tra