Cloning and molecular analysis of a cDNA and the Cs-mnpl gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete Ceriporiopsis subvermispora
Author
dc.contributor.author
Montané Lobos, Sergio Luis Andrés
Author
dc.contributor.author
Larrondo, Luis F.
Author
dc.contributor.author
Salas, Loreto
Author
dc.contributor.author
Karahanian, Eduardo
Author
dc.contributor.author
Vicuña, Rafael
Admission date
dc.date.accessioned
2019-01-29T17:16:07Z
Available date
dc.date.available
2019-01-29T17:16:07Z
Publication date
dc.date.issued
1998
Cita de ítem
dc.identifier.citation
Gene, Volumen 206, Issue 2, 2018, Pages 185-193
Identifier
dc.identifier.issn
03781119
Identifier
dc.identifier.other
10.1016/S0378-1119(97)00583-0
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/163410
Abstract
dc.description.abstract
A cDNA (MnP13-1) and the Cs-mnp1 gene encoding for an isoenzyme of manganese peroxidase (MnP) from C. subvermispora were isolated separately and sequenced. The cDNA, identified in a library constructed in the vector Lambda ZIPLOX, contains 1285 nucleotides, excluding the poly(A) tail, and has a 63% G+C content. The deduced protein sequence shows a high degree of identity with MnPs from other fungi. The mature protein contains 364 amino acids, which are preceded by a 24-amino-acid leader sequence. Consistent with the peroxidase mechanism of MnP, the proximal histidine, the distal histidine and the distal arginine are conserved, although the aromatic binding site (L/V/I-P-X-P) is less hydrophilic than those of other peroxidases. A gene coding for the same protein (Cs-mnpl) was isolated from a genomic library constructed in Lambda GEM-11 vector using the cDNA MnP13-1 as a probe. A subcloned SacI fragment of 2.5 kb contained the complete sequence of the Cs-mnpl gene, including 162 bp and 7
Cloning and molecular analysis of a cDNA and the Cs-mnpl gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete Ceriporiopsis subvermispora