Augmented cell survival in eutopic endometrium from women with endometriosis: Expression of c-myc, TGF-beta1 and bax genes
Author
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Johnson, M. Cecilia
Author
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Torres, Marisa
Author
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Alves, Alessandra
Author
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Bacallao, Ketty
Author
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Fuentes, Ariel
Author
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Vega Blanco, María Margarita
Author
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Boric Scarpa, María Angélica
Admission date
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2019-01-29T17:57:15Z
Available date
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2019-01-29T17:57:15Z
Publication date
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2005
Cita de ítem
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Reproductive Biology and Endocrinology, Volumen 3,
Identifier
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14777827
Identifier
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14777827
Identifier
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10.1186/1477-7827-3-45
Identifier
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https://repositorio.uchile.cl/handle/2250/163968
Abstract
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Background: Endometriosis is a common gynaecological disorder characterized by the presence of endometrial tissue outside of the uterus. The fragments in normal menstruation are composed of necrotic and living cells, which do not survive in ectopic locations because of programmed cell death. The aim of this study was to evaluate if the balance between cell proliferation and apoptosis is changed in eutopic endometrium from women with endometriosis throughout the menstrual cycle by studying bax (pro-apoptotic), c-myc (regulator of cell cycle) and TGF-betal (involved in cell differentiation) genes. Methods: Eutopic endometrium was obtained from: 30 women with endometriosis (32.8 +/- 5 years) and 34 fertile eumenorrheic women (36 +/- 5.3 years). We analyzed apoptosis (TUNEL: DNA fragmentation); cell proliferation (immunohistochemistry (IHC) for Ki67); c-myc, bax and TGF-betal mRNA abundance (RT-PCR) and TGF-betal protein (IHC) in endometrial explants. Results: Cell proliferation strongly d