Predicting the behaviour of proteins in hydrophobic interaction chromatography: 2. Using a statistical description of their surface amino acid distribution
Author
dc.contributor.author
Salgado, J. Cristian
Author
dc.contributor.author
Rapaport Zimermann, Iván
Author
dc.contributor.author
Asenjo de Leuze, Juan
Admission date
dc.date.accessioned
2019-01-29T17:57:16Z
Available date
dc.date.available
2019-01-29T17:57:16Z
Publication date
dc.date.issued
2006
Cita de ítem
dc.identifier.citation
Journal of Chromatography A, Volumen 1107, Issue 1-2, 2018, Pages 120-129
Identifier
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00219673
Identifier
dc.identifier.other
10.1016/j.chroma.2005.12.033
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/163977
Abstract
dc.description.abstract
This paper focuses on the prediction of the dimensionless retention time (DRT) of proteins in hydrophobic interaction chromatography (HIC) by means of mathematical models based on the statistical description of the amino acid surface distribution. Previous models characterises the protein surface as a whole. However, most of the time it is not the whole protein but some of its specific regions that interact with the environment. It seems much more natural to use local measurements of the characteristics of the surface. Therefore, the statistical characterisation of the distribution of an amino acid property on the protein surface was carried out from the systematic calculation of the local average of this property in a neighbourhood placed sequentially on each of the amino acids on the protein surface. This process allowed us to characterise the distribution of this property quantitatively using three main statistics: average, standard deviation and maximum. In particular, if the prope
Predicting the behaviour of proteins in hydrophobic interaction chromatography: 2. Using a statistical description of their surface amino acid distribution