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Membrane electrical activity elicits inositol 1,4,5-trisphosphate-dependent slow Ca2+ signals through a Gβγ/phosphatidylinositol 3-kinase γ pathway in skeletal myotubes

Authordc.contributor.authorEltit Ortega, José Miguel 
Authordc.contributor.authorGarcía, Alejandra 
Authordc.contributor.authorHidalgo, Jorge 
Authordc.contributor.authorLiberona, José L. 
Authordc.contributor.authorChiong Lay, Mario 
Authordc.contributor.authorLavandero González, Sergio 
Authordc.contributor.authorMaldonado, Edio 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Admission datedc.date.accessioned2019-03-11T12:51:09Z
Available datedc.date.available2019-03-11T12:51:09Z
Publication datedc.date.issued2006
Cita de ítemdc.identifier.citationJournal of Biological Chemistry, Volumen 281, Issue 17, 2018, Pages 12143-12154
Identifierdc.identifier.issn1083351X
Identifierdc.identifier.issn00219258
Identifierdc.identifier.other10.1074/jbc.M511218200
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/164158
Abstractdc.description.abstractTetanic electrical stimulation of myotubes evokes a ryanodine receptor-related fast calcium signal, during the stimulation, followed by a phospholipase C/inositol 1,4,5-trisphosphate-dependent slow calcium signal few seconds after stimulus end. L-type calcium channels (Cav 1.1, dihydropyridine receptors) acting as voltage sensors activate an unknown signaling pathway involved in phospholipase C activation. We demonstrated that both G protein and phosphatidylinositol 3-kinase were activated by electrical stimulation, and both the inositol 1,4,5-trisphosphate rise and slow calcium signal induced by electrical stimulation were blocked by pertussis toxin, by a Gβγ scavenger peptide, and by phosphatidylinositol 3-kinase inhibitors. Immunofluorescence using anti-phosphatidylinositol 3-kinase γ antibodies showed a clear location in striations within the cytoplasm, consistent with a position near the I band region of the sarcomere. The time course of phosphatidylinositol 3-kinase activation, m
Lenguagedc.language.isoen
Publisherdc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Biological Chemistry
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectCell Biology
Títulodc.titleMembrane electrical activity elicits inositol 1,4,5-trisphosphate-dependent slow Ca2+ signals through a Gβγ/phosphatidylinositol 3-kinase γ pathway in skeletal myotubes
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile