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Permeability of human jejunal segments to gonyautoxins measured by the Ussing chamber technique

Authordc.contributor.authorMardones, Pamela 
Authordc.contributor.authorAndrinolo, Darío 
Authordc.contributor.authorCsendes Juhasz, Attila 
Authordc.contributor.authorLagos, Néstor 
Admission datedc.date.accessioned2019-03-11T12:55:55Z
Available datedc.date.available2019-03-11T12:55:55Z
Publication datedc.date.issued2004
Cita de ítemdc.identifier.citationToxicon, Volumen 44, Issue 5, 2018, Pages 521-528
Identifierdc.identifier.issn00410101
Identifierdc.identifier.other10.1016/j.toxicon.2004.07.003
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/164555
Abstractdc.description.abstractThe aim of this work was to study the mechanisms involved in intestinal permeability of gonyautoxins. For this purpose, the influence on transmucosal resistance of gonyautoxins and their permeability was investigated in excised human jejunal segments. To evaluate these events, the isolated mucosa was mounted in Ussing chambers for electrophysiological characterization. The organic gonyautoxin cations were applied to the mucosal side and samples collected on the serosal side. The permeability of gonyautoxins measured at 37°C was 4.3-fold greater than at 4°C, indicative of high cation selective transcellular permeability. In order to characterize the permeability of gonyautoxins, the effects of choline, ouabain, phlorizin and fluorescein were studied. The inhibition by these compounds was expressed as percent inhibition of the maximal flux of gonyautoxins at 120 min. Replacement of sodium ion by choline, showed the highest inhibition (85.5% from control). Ouabain, fluorescein and phloriz
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceToxicon
Keywordsdc.subjectGonyautoxins
Keywordsdc.subjectMannitol
Keywordsdc.subjectOuabain
Keywordsdc.subjectPermeability
Keywordsdc.subjectPhlorizin
Keywordsdc.subjectUssing chamber
Títulodc.titlePermeability of human jejunal segments to gonyautoxins measured by the Ussing chamber technique
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile