The linear plasmid prophage Vp58.5 of Vibrio parahaemolyticus is closely related to the integrating phage VHML and constitutes a new incompatibility group of telomere phages
Author
dc.contributor.author
Zabala, Beatriz
Author
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Hammerl, Jens A.
Author
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Espejo Torres, Romilio
Author
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Hertwig, Stefan
Admission date
dc.date.accessioned
2019-03-11T12:58:10Z
Available date
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2019-03-11T12:58:10Z
Publication date
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2009
Cita de ítem
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Journal of Virology, Volumen 83, Issue 18, 2018, Pages 9313-9320
Identifier
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0022538X
Identifier
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10.1128/JVI.00672-09
Identifier
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https://repositorio.uchile.cl/handle/2250/164824
Abstract
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Vibrio parahaemolyticus O3:K6 pandemic strains recovered in Chile frequently possess a 42-kb plasmid which is the prophage of a myovirus. We studied the prototype phage VP58.5 and show that it does not integrate into the host cell chromosome but replicates as a linear plasmid (Vp58.5) with covalently closed ends (telomeres). The Vp58.5 replicon coexists with other plasmid prophages (N15, PY54, and ΦKO2) in the same cell and thus belongs to a new incompatibility group of telomere phages. We determined the complete nucleotide sequence (42,612 nucleotides) of the VP58.5 phage DNA and compared it with that of the plasmid prophage. The two molecules share the same nucleotide sequence but are 35% circularly permuted to each other. In contrast to the hairpin ends of the plasmid, VP58.5 phage DNA contains 5′-protruding ends. The VP58.5 sequence is 92% identical to the sequence of phage VHML, which was reported to integrate into the host chromosome. However, the gene order and termini of the ph
The linear plasmid prophage Vp58.5 of Vibrio parahaemolyticus is closely related to the integrating phage VHML and constitutes a new incompatibility group of telomere phages