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tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori

Authordc.contributor.authorCerda, Oscar A. 
Authordc.contributor.authorNúñez-Villena, Felipe 
Authordc.contributor.authorSoto, Sarita E. 
Authordc.contributor.authorUgalde, José Manuel 
Authordc.contributor.authorLópez Solís, Remigio 
Authordc.contributor.authorToledo, Héctor 
Admission datedc.date.accessioned2019-03-11T13:02:33Z
Available datedc.date.available2019-03-11T13:02:33Z
Publication datedc.date.issued2011
Cita de ítemdc.identifier.citationBiological Research, Volumen 44, Issue 3, 2018, Pages 277-282
Identifierdc.identifier.issn07169760
Identifierdc.identifier.issn07176287
Identifierdc.identifier.other10.4067/S0716-97602011000300009
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/165401
Abstractdc.description.abstractAbout half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer. Chemotaxis and flagellar motility are required for colonization and persistence of H. pylori in the gastric mucus layer. It is not completely clear which chemical gradients are used by H. pylori to maintain its position. TlpA, a chemotaxis receptor for arginine/ bicarbonate, has been identified. This study aimed to find out whether tlpA gene expression is required for the chemotactic response to arginine/bicarbonate. Wild-type motile H. pylori ATCC 700392 and H. pylori ATCC 43504, a strain having an interrupted tlpA gene, were used. Also, a tlpA-knockout mutant of H. pylori 700392 (H. pylori 700-tlpA::cat) was produced by homologous recombination. Expression of tlpA was assessed by a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assay. Chemotaxis was measured as a Relative Chemotaxis Response (RCR) by a modified capillary ass
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceBiological Research
Keywordsdc.subjectArginine
Keywordsdc.subjectBicarbonate
Keywordsdc.subjectChemotaxis
Keywordsdc.subjectHelicobacter pylori
Keywordsdc.subjectTlpa
Títulodc.titletlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile