Exercise sensitizes skeletal muscle to extracellular ATP for IL-6 expression in mice
Author
dc.contributor.author
Fernández Verdejo, Rodrigo
Author
dc.contributor.author
Casas, M.
Author
dc.contributor.author
Galgani Fuentes, José
Author
dc.contributor.author
Jaimovich Pérez, Enrique
Author
dc.contributor.author
Buvinic Radic, Sonja
Admission date
dc.date.accessioned
2019-03-15T16:06:12Z
Available date
dc.date.available
2019-03-15T16:06:12Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
International Journal of Sports Medicine, Volumen 35, Issue 4, 2018, Pages 273-279
Identifier
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14393964
Identifier
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01724622
Identifier
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10.1055/s-0033-1353147
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/166140
Abstract
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Active skeletal muscle synthesizes and releases interleukin-6 (IL-6), which plays important roles in the organism's adaptation to exercise. Autocrine/paracrine ATP signaling has been shown to modulate IL-6 expression. The aim of this study was to determine whether a period of physical activity modifies the ATP-induced IL-6 expression. BalbC mice were either subject to 5 weeks voluntary wheel running (VA) or kept sedentary (SED). Flexor digitorum brevis muscles were dissected, stimulated with different ATP concentrations (0-100 μM) and IL-6 mRNA levels were measured using qPCR. ATP evoked a concentration-dependent rise in IL-6 mRNA in both SED and VA mice. VA mice however, had significantly higher ATP sensitivity (pD2 pharmacological values: VA=5.58±0.02 vs. SED=4.95±0.04, p<0.05). Interestingly, in VA mice we observed a positive correlation between the level of physical activity and the IL-6 mRNA increase following fiber stimulation with 10μM ATP. In addition, there were lower P2Y2- an