A novel immunomodulatory hemocyanin from the limpet Fissurella latimarginata promotes potent anti-tumor activity in melanoma
Author
dc.contributor.author
Arancibia, Sergio
Author
dc.contributor.author
Espinoza, Cecilia
Author
dc.contributor.author
Salazar, Fabián
Author
dc.contributor.author
Del Campo, Miguel
Author
dc.contributor.author
Tampe, Ricardo
Author
dc.contributor.author
Zhong, Ta Ying
Author
dc.contributor.author
Ioannes, Pablo de
Author
dc.contributor.author
Moltedo, Bruno
Author
dc.contributor.author
Ferreira, Jorge
Author
dc.contributor.author
Lavelle, Ed C.
Author
dc.contributor.author
Manubens, Augusto
Author
dc.contributor.author
Ioannes, Alfredo de
Author
dc.contributor.author
Becker, María Inés
Admission date
dc.date.accessioned
2019-03-15T16:06:33Z
Available date
dc.date.available
2019-03-15T16:06:33Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
PLoS ONE, Volumen 9, Issue 1, 2018,
Identifier
dc.identifier.issn
19326203
Identifier
dc.identifier.other
10.1371/journal.pone.0087240
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/166149
Abstract
dc.description.abstract
Hemocyanins, the huge oxygen-transporting glycoproteins of some mollusks, are used as immunomodulatory proteins with proven anti-cancer properties. The biodiversity of hemocyanins has promoted interest in identifying new anti-cancer candidates with improved immunological properties. Hemocyanins promote Th1 responses without known side effects, which make them ideal for long-term sustained treatment of cancer. In this study, we evaluated a novel hemocyanin from the limpet/gastropod Fissurella latimarginata (FLH). This protein has the typical hollow, cylindrical structure of other known hemocyanins, such as the keyhole limpet hemocyanin (KLH) and the Concholepas hemocyanin (CCH). FLH, like the KLH isoforms, is composed of a single type of polypeptide with exposed N- and O-linked oligosaccharides. However, its immunogenicity was significantly greater than that of KLH and CCH, as FLH induced a stronger humoral immune response and had more potent anti-tumor activity, delaying tumor growth a