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Crohn associated microbial communities associated to colonic mucosal biopsies in patients of the western Mediterranean

Authordc.contributor.authorVidal, Roberto 
Authordc.contributor.authorGinard, Daniel 
Authordc.contributor.authorKhorrami, Sam 
Authordc.contributor.authorMora-Ruiz, Merit 
Authordc.contributor.authorMunoz, Raul 
Authordc.contributor.authorHermoso, Marcela 
Authordc.contributor.authorDíaz, Sara 
Authordc.contributor.authorCifuentes, Ana 
Authordc.contributor.authorOrfila, Alejandro 
Authordc.contributor.authorRosselló-Móra, Ramon 
Cita de ítemdc.identifier.citationSystematic and Applied Microbiology, Volumen 38, Issue 6, 2018, Pages 442-452
Abstractdc.description.abstract© 2015 Elsevier GmbH. Next generation sequencing approaches allow the retrieval of several orders of magnitude larger numbers of amplified single sequences in 16S rRNA diversity surveys than classical methods. However, the sequences are only partial and thus lack sufficient resolution for a reliable identification. The OPU approach used here, based on a tandem combination of high quality 454 sequences (mean >500 nuc) applying strict OTU thresholds, and phylogenetic inference based on parsimony additions to preexisting trees, seemed to improve the identification yields at the species and genus levels. A total of thirteen biopsies of Crohn-diagnosed patients (CD) and seven healthy controls (HC) were studied. In most of the cases (73%), sequences were affiliated to known species or genera and distinct microbial patterns could be distinguished among the CD subjects, with a common depletion of Clostridia and either an increased presence of Bacteroidetes (CD1) or an anomalous overrepresentat
Publisherdc.publisherElsevier GmbH
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceSystematic and Applied Microbiology
Keywordsdc.subjectCrohn disease
Keywordsdc.subjectFaecalibacterium prausnitzii
Keywordsdc.subjectGut microbiome
Keywordsdc.subjectOperational phylogenetic units
Títulodc.titleCrohn associated microbial communities associated to colonic mucosal biopsies in patients of the western Mediterranean
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile