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Authordc.contributor.authorCruz, P. 
Authordc.contributor.authorSosoniuk Roche, E. 
Authordc.contributor.authorMaldonado, I. 
Authordc.contributor.authorTorres Mendoza, Cristián G. 
Authordc.contributor.authorFerreira, A. 
Admission datedc.date.accessioned2020-06-06T21:12:56Z
Available datedc.date.available2020-06-06T21:12:56Z
Publication datedc.date.issued2020
Cita de ítemdc.identifier.citationImmunobiology 225 (2020) 151892es_ES
Identifierdc.identifier.other10.1016/j.imbio.2019.12.001
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/175290
Abstractdc.description.abstractRecombinant calreticulin from Trypanosoma cruzi (rTcCalr), the parasite responsible for Chagas' disease, binds to Canine Transmissible Venereal Tumor (CTVT) cells from primary cultures and to a canine mammary carcinoma cell line. A Complement-binding assay indicated that interaction of the first component C1q with these tumor cells operated independently of the rTcCalr-presence. This apparent independence could be explained by the important structural similarities that exist among rTcCarl, endogenous normal canine and/or mutated calreticulins present in several types of cancer. In phagocytosis assays, tumor cells treated with rTcCalr were readily engulfed by macrophages and, co-cultured with DCs, accelerated their maturation. In addition, DCs maturation, induced by tumor cells co-cultured with rTcCalr, activated T cells more efficiently than DCs, treated or not with LPS. In an apparent paradox, a decrease in MHC Class I expression was observed when these tumor cells were cocultivated with rTcCalr. This decrease may be related to a down regulation signaling promoting the rescue of MHC I. Possibly, these in vitro assays may be valid correlates of in vivo sceneries. Based on these results, we propose that rTcCalr improves in vitro the immunogenicity of two widely different tumor cell lines, thus suggesting that the interesting properties of rTcCalr to boost immune responses warrant future studies.es_ES
Patrocinadordc.description.sponsorshipComisión Nacional de Investigación Científica y Tecnológica (CONICYT) CONICYT FONDECYT 1130099 Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) 21150960 Proyecto Enlace, VID, Universidad de Chile, 2017-2018es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherElsevieres_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceImmunobiologyes_ES
Keywordsdc.subjectCalreticulines_ES
Keywordsdc.subjectCTVT cellses_ES
Keywordsdc.subjectTrypanosoma cruzies_ES
Keywordsdc.subjectTumor cellses_ES
Keywordsdc.subjectDendritic cellses_ES
Keywordsdc.subjectT cellses_ES
Títulodc.titleTrypanosoma cruzi calreticulin: In vitro modulation of key immunogenic markers of both canine tumors and relevant immune competent cellses_ES
Document typedc.typeArtículo de revistaes_ES
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Catalogueruchile.catalogadorctces_ES
Indexationuchile.indexArtículo de publicación ISI
Indexationuchile.indexArtículo de publicación SCOPUS


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile