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Modelación Computacional de Esfuerzos y Deformaciones en el Fenómeno de Brain Shift
(Universidad de Chile, 2011)
Identificación de bacterias con potencial actividad anticancerígena a partir de muestras de la fosa de Atacama
(Universidad de Chile, 2022)
analizadas por investigadores del Centro de Biotecnología y Bioingeniería (CeBiB), quienes lograron el aislamiento de cepas de Actinobacterias que poseen un gran valor biotecnológico.
Los objetivos de este trabajo son evaluar la potencial actividad...
Cultivo de hongos marinos a partir de residuos de la industria algal rica en alginato
(Universidad de Chile, 2018)
Implante de células troncales adultas derivadas del tejido adiposo en una matriz dérmica acelular (integra) para optimizar la cicatrización en áreas desprovistas de piel
(Universidad de Chile, 2011)
El tratamiento quirúrgico de las lesiones que incluyen pérdida de tejido cutáneo ha presentado importantes innovaciones con el advenimiento de la medicina regenerativa y la bioingeniería tisular, esto ha incluido dentro de las alternativas...
Surgical treatment of injuries with loss of skin tissue has made significant inno-vations with the advent of regenerative medicine and tissue bioengineering, this has included the use of stem cell and dermal substitutes. The success of tissue regenera-tion depends on an optimal local vascularization, in addition to the successful integra-tion of the artificial skin. The present study combines the use of autologous adipose tissue derived stem cells derived (ASCs) with a commercially available dermal substi-tute (Integra ®) for skin regeneration. The ASCs obtained from the inguinal region of 8 adult rats are seeded in a piece of dermal substitute for 48 hours, and then im-planted in the same rat, comparing the evolution with a contralateral implant without ASCs. After 1, 2 and 3 weeks of regeneration in vivo, implants were removed for his-tological evaluation. Our results show that the implants are properly integrated with autologous tissue, the ASCs significantly increased microvascular density (7.7 ± 0.6% vs. 5.3 ± 0.5%, assessed by immunohistochemical staining anti-factor VIII) and the synthesis of type I collagen (24 ± 3% vs. 16 ± 2%, assessed by Junqueira Sirius red staining). While there was a greater epithelialisation on the side treated with ASCs (2530 vs. 1911 microns), assessed by anti-cytokeratin immunohistochemistry staining 34βE12, this difference was not statistically significant. Not identified differentiation of new epidermal cells in the time period studied. Thus, our data suggest that seeding ASCs in a dermal substitute improve skin regeneration and tissue integration by in-creasing vascularity and collagen synthesis....
Surgical treatment of injuries with loss of skin tissue has made significant inno-vations with the advent of regenerative medicine and tissue bioengineering, this has included the use of stem cell and dermal substitutes. The success of tissue regenera-tion depends on an optimal local vascularization, in addition to the successful integra-tion of the artificial skin. The present study combines the use of autologous adipose tissue derived stem cells derived (ASCs) with a commercially available dermal substi-tute (Integra ®) for skin regeneration. The ASCs obtained from the inguinal region of 8 adult rats are seeded in a piece of dermal substitute for 48 hours, and then im-planted in the same rat, comparing the evolution with a contralateral implant without ASCs. After 1, 2 and 3 weeks of regeneration in vivo, implants were removed for his-tological evaluation. Our results show that the implants are properly integrated with autologous tissue, the ASCs significantly increased microvascular density (7.7 ± 0.6% vs. 5.3 ± 0.5%, assessed by immunohistochemical staining anti-factor VIII) and the synthesis of type I collagen (24 ± 3% vs. 16 ± 2%, assessed by Junqueira Sirius red staining). While there was a greater epithelialisation on the side treated with ASCs (2530 vs. 1911 microns), assessed by anti-cytokeratin immunohistochemistry staining 34βE12, this difference was not statistically significant. Not identified differentiation of new epidermal cells in the time period studied. Thus, our data suggest that seeding ASCs in a dermal substitute improve skin regeneration and tissue integration by in-creasing vascularity and collagen synthesis....