Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous
Artículo
Open/ Download
Access note
Acceso Abierto
Publication date
2011Metadata
Show full item record
Cómo citar
Martínez Moya, Pilar
Cómo citar
Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous
Author
Abstract
Background: The yeast Xanthophyllomyces dendrorhous is used for the microbiological production of the antioxidant carotenoid astaxanthin. In this study, we established an optimal protocol for protein extraction and performed the first proteomic analysis of the strain ATCC 24230. Protein profiles before and during the induction of carotenogenesis were determined by two-dimensional polyacrylamide gel electrophoresis and proteins were identified by mass spectrometry. Results: Among the approximately 600 observed protein spots, 131 non-redundant proteins were identified. Proteomic analyses allowed us to identify 50 differentially expressed proteins that fall into several classes with distinct expression patterns. These analyses demonstrated that enzymes related to acetyl-CoA synthesis were more abundant prior to carotenogenesis. Later, redox- and stress-related proteins were up-regulated during the induction of carotenogenesis. For the carotenoid biosynthetic enzymes mevalonate kinase and
Indexation
Artículo de publicación SCOPUS
Identifier
URI: https://repositorio.uchile.cl/handle/2250/154833
DOI: 10.1186/1471-2180-11-131
ISSN: 14712180
14712180
Quote Item
BMC Microbiology, Volumen 11,
Collections