Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel
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2001Metadata
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Cornejo, Isabel
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Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel
Abstract
We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3 - > Cl- > Br- > I- > F- > gluconate-. The possible role of gpClC-5 in this epithelial location is discussed. © 2001 Elsevier Science B.V.
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URI: https://repositorio.uchile.cl/handle/2250/163486
DOI: 10.1016/S0005-2736(01)00331-5
ISSN: 00052736
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Biochimica et Biophysica Acta - Biomembranes, Volumen 1512, Issue 2, 2018, Pages 367-374
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