Real-Time H2O2 Measurements in Bone Marrow Mesenchymal Stem Cells (MSCs) Show Increased Antioxidant Capacity in Cells From Osteoporotic Women
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Román, Flavia
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Real-Time H2O2 Measurements in Bone Marrow Mesenchymal Stem Cells (MSCs) Show Increased Antioxidant Capacity in Cells From Osteoporotic Women
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© 2016 Wiley Periodicals, Inc. Oxidative stress (OS) derived from an increase in intracellular reactive oxygen species (ROS) is a major determinant of aging and lifespan. It has also been associated with several age-related disorders, like postmenopausal osteoporosis of Mesenchymal stem cells (MSCs). MSCs are the common precursors for osteoblasts and adipocytes; appropriate commitment and differentiation of MSCs into a specific phenotype is modulated, among other factors, by ROS balance. MSCs have shown more resistance to ROS than differentiated cells, and their redox status depends on complex and abundant anti-oxidant mechanisms. The purpose of this work was to analyze in real time, H2O2 signaling in individual h-MSCs, and to compare the kinetic parameters of H2O2 management by cells derived from both control (c-) and osteoporotic (o-) women. For these purposes, cells were infected with a genetically encoded fluorescent biosensor named HyPer, which is specific for detecting H2O2 insid
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URI: https://repositorio.uchile.cl/handle/2250/166910
DOI: 10.1002/jcb.25739
ISSN: 10974644
07302312
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Journal of Cellular Biochemistry, Volumen 118, Issue 3, 2018, Pages 585-593
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