The β-isoform of heat shock protein hsp-90 is structurally related with human microtubule-interacting protein Mip-90
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Cambiazo Ayala, Verónica
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The β-isoform of heat shock protein hsp-90 is structurally related with human microtubule-interacting protein Mip-90
Abstract
Through major research advances in the study of cytoskeletal organization, an integrated view of the complexity of this system has emerged. Recent findings on the microtubule-interacting protein Mip-90, which associates with microtubules and actin filaments in different cell domains, have shed light on its roles in cytoskeletal regulation. In order to study structural features of Mip-90, we sequenced several peptide fragments. A comparative sequence analysis revealed a high degree of similarity between the primary structure of this protein and the human heat shock protein of 90 kDa (hsp-90). Taken together, the present studies indicate the identity between Mip-90 and the the β-isoform of hsp-90 (hsp-90β). Western blot assays with an anti-hsp-90 monoclonal antibody showed cross-reactivity of hsp-90 and Mip-90 affinity purified from HeLa cells. Furthermore, the observed structural identity of Mip-90 with the hsp-90β was sustained by immunoblot assays using monoclonal antibodies that spec
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URI: https://repositorio.uchile.cl/handle/2250/154396
DOI: 10.1016/S0014-5793(99)01070-4
ISSN: 00145793
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FEBS Letters, Volumen 457, Issue 3, 2018, Pages 343-347
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