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T-kininogen, a cystatin-like molecule, inhibits ERK-dependent lymphocyte proliferation

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2005-12
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Acuña Castillo, Claudio
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T-kininogen, a cystatin-like molecule, inhibits ERK-dependent lymphocyte proliferation
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Author
  • Acuña Castillo, Claudio;
  • Aravena, Mauricio;
  • Leiva Salcedo, Elías;
  • Pérez, Viviana;
  • Gómez, Christian;
  • Sabaj Diez, Valeria;
  • Nishimura, Sumiyo;
  • Pérez Núñez, Claudio;
  • Colombo, Alicia;
  • Walter, Robin Ann;
  • Sierra, Felipe;
Abstract
Plasma levels of kininogens increase with age in both rats and humans. Kininogens are inhibitors of cysteine proteinases, and filarial cysteine proteinase inhibitors (cystatins) reduce the proliferation of T cells. We evaluated whether T-kininogen (T-KG) might mimic this effect, and here we present data indicating that exposure of either rat splenocytes or Jurkat cells to purified T-KG results in inhibition of both ERK activation and [H-3]-thymidine incorporation, both basal and in response to ConA or PHA. Interestingly, T-KG did not impair [H-3]-thymidine incorporation in response to IL-2, which requires primarily the activation of the JNK and Jak/STAT pathways. These effects were neither the consequence of increased cell death, nor required the activity of kinin receptors. Furthermore, when T cell receptor proximal events were bypassed by the use of PMA plus Calcium ionophore, T-KG no longer inhibited ERK activation, suggesting that inhibition occurs upstream of these events, possibly at the level of membrane associated signal transduction molecules. We conclude that, like filarial cystatins, T-KG inhibits ERK-dependent T cell proliferation, and these observations suggest a possible role for T-KG in immunosenescence.
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URI: https://repositorio.uchile.cl/handle/2250/127142
ISSN: 0047-6374
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MECHANISMS OF AGEING AND DEVELOPMENT 126 (12): 1284-1291 DEC 2005
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